A consortium of rhizobacterial strains and biochemical growth elicitors improve cold and drought stress tolerance in rice (Oryza sativa L.)

In the present study, a consortium of two rhizobacteria Bacillus amyloliquefaciens Bk7 and Brevibacillus laterosporus B4, termed 'BB', biochemical elicitors salicylic acid and β-aminobutyric acid (SB) and their mixture (BBSB) were investigated for cold and drought stress tolerance in rice...

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Published in:Plant biology (Stuttgart, Germany) Germany), 2016-05, Vol.18 (3), p.471-483
Main Authors: Kakar, K. U., Ren, X.-l., Nawaz, Z., Cui, Z.-Q., Li, B., Xie, G.-L., Hassan, M. A., Ali, E., Sun, G.-C.
Format: Article
Language:English
Subjects:
Aminobutyrates - metabolism
Antioxidants
Antioxidants - metabolism
Bacillus amyloliquefaciens - physiology
bio-formulation
Biochemical growth elicitors
Biofilms
Brevibacillus - physiology
Catalase
Catalase - metabolism
Chlorosis
cold and drought stress
Cold Temperature
Cold tolerance
Consortia
Drought
Droughts
Electrolyte leakage
Formulations
Gene expression
Genes
induced resistance
Inoculation
Leaves
Oryza - drug effects
Oryza - enzymology
Oryza - physiology
Oryza sativa
Plant growth
Plant Leaves - drug effects
Plant Leaves - enzymology
Plant Leaves - physiology
Plant roots
Plant Roots - drug effects
Plant Roots - enzymology
Plant Roots - physiology
Proline
Proline - metabolism
Rice
Salicylic acid
Salicylic Acid - metabolism
Seedlings
Siderophores
Siderophores - metabolism
Strains (organisms)
Stress
Stress, Physiological
Stresses
Superoxide dismutase
Superoxide Dismutase - metabolism
Water - metabolism
Water treatment
Wilting
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Summary:In the present study, a consortium of two rhizobacteria Bacillus amyloliquefaciens Bk7 and Brevibacillus laterosporus B4, termed 'BB', biochemical elicitors salicylic acid and β-aminobutyric acid (SB) and their mixture (BBSB) were investigated for cold and drought stress tolerance in rice plants. After withholding water for 16 days, rice plants treated with BBSB showed 100% survival, improved seedling height (35.4 cm), shoot number (6.12), and showed minimum symptoms of chlorosis (19%), wilting (4%), necrosis (6%) and rolling of leaves. Similarly, BB inoculation enhanced plant growth and reduced overall symptoms in rice seedlings subjected to 0 ± 5 °C for 24 h. Our results imply several mechanisms underlying BB- and BBSB-elicited stress tolerance. In contrast to the control, both treatments significantly decreased leaf monodehydroascorbate (MDA) content and electrolyte leakage, and increased leaf proline and cholorophyll content. Moreover, activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) increased 3.0- and 3.6-fold, respectively. Moreover, expression of OsMYB3R-2, OsDIL, OsDREB1A and OsCDPK13 genes was significantly up-regulated, suggesting that these genes play important roles in abiotic stress tolerance of rice. In addition, bacterial strains Bk7 and B4 were able to produce high amounts of IAA and siderophores, and colonise the plant roots, while only strain Bk7 exhibited the capability to form biofilms and solubilise inorganic phosphate. This study indicates that the BB and BBSB bio-formulations can be used to confer induced systematic tolerance and improve the health of rice plants subject to chilling and drought stress.
ISSN:1435-8603
1438-8677
DOI:10.1111/plb.12427