HPV prevalence and type-distribution in cervical cancer and premalignant lesions of the cervix: A population-based study from Northern Ireland

Assessment of Human papillomavirus (HPV) prevalence and genotype distribution is important for monitoring the impact of prophylactic HPV vaccination. This study aimed to demonstrate the HPV genotypes predominating in pre‐malignant and cervical cancers in Northern Ireland (NI) before the vaccination...

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Bibliographic Details
Published in:Journal of medical virology 2016-07, Vol.88 (7), p.1262-1270
Main Authors: Anderson, Lesley A., O'Rorke, Michael A., Wilson, Robbie, Jamison, Jackie, Gavin, Anna T.
Format: Article
Language:English
Subjects:
Adenocarcinoma - epidemiology
Adenocarcinoma - virology
Adult
Aged
Carcinoma, Squamous Cell - epidemiology
Carcinoma, Squamous Cell - virology
Cervical cancer
cervical intraepithelial neoplasia
Cervical Intraepithelial Neoplasia - epidemiology
Cervical Intraepithelial Neoplasia - virology
Cervix Uteri - pathology
Cervix Uteri - virology
DNA, Viral - analysis
Epidemiology
Female
frequency
Genotype
Genotype & phenotype
Human papillomavirus
Human papillomavirus 16 - classification
Human papillomavirus 16 - genetics
Human papillomavirus 16 - isolation & purification
Human papillomavirus 18 - classification
Human papillomavirus 18 - genetics
Human papillomavirus 18 - isolation & purification
Humans
Ireland - epidemiology
Northern Ireland - epidemiology
Papillomavirus Infections - epidemiology
Papillomavirus Infections - virology
Polymerase Chain Reaction
population-based
Prevalence
Risk Factors
Uterine Cervical Neoplasms - epidemiology
Uterine Cervical Neoplasms - virology
Virology
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Summary:Assessment of Human papillomavirus (HPV) prevalence and genotype distribution is important for monitoring the impact of prophylactic HPV vaccination. This study aimed to demonstrate the HPV genotypes predominating in pre‐malignant and cervical cancers in Northern Ireland (NI) before the vaccination campaign has effect. Formalin fixed paraffin embedded tissue blocks from 2,303 women aged 16–93 years throughout NI were collated between April 2011 and February 2013. HPV DNA was amplified by PCR and HPV genotyping undertaken using the Roche® linear array detection kit. In total, 1,241 out of 1,830 eligible samples (68.0%) tested positive for HPV, with the majority of these [1,181/1,830 (64.5%)] having high‐risk (HR) HPV infection; 37.4% were positive for HPV‐16 (n = 684) and 5.1% for HPV‐18 (n = 93). HPV type‐specific prevalence was 48.1%, 65.9%, 81.3%, 92.2%, and 64.3% among cervical intraepithelial neoplasias (CIN) Grades I–III, squamous cell carcinomas (SCC) and adenocarcinoma (AC) cases, respectively. Most SCC cases (81.3%) had only one HPV genotype detected and almost a third (32.0%) of all cervical pathologies were HPV negative including 51.9% of CIN I (n = 283), 34.1% CIN II (n = 145), 18.7% of CIN III (n = 146), 7.8% of SCC (n = 5), and 35.7% of AC (n = 5) cases. This study provides important baseline data for monitoring the effect of HPV vaccination in NI and for comparison with other UK regions. The coverage of other HR‐HPV genotypes apart from 16 and 18, including HPV‐45, 31, 39, and 52, and the potential for cross protection, should be considered when considering future polyvalent vaccines. J. Med. Virol. 88:1262–1270, 2016. © 2015 Wiley Periodicals, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.24447