Abnormal Morphological Vesicles in Influenza a Virus Exposed to Acid pH

Vesicles on the virion surface, which continued the lipoprotein membrane but had no spikes of virus glycoproteins hemagglutinin (HA) and neuraminidase (NA), were detected. These vesicles and virus particles were 18±7 and 103±12 nm in diameter, respectively, and, as a rule, one vesicle was found per...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2015-04, Vol.158 (6), p.776-780
Main Authors: Zhirnov, O. P., Manykin, A. A.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Dogs
Glycoproteins
Hemagglutinins - metabolism
Hydrogen-Ion Concentration
Influenza
Influenza A virus
Influenza A virus - metabolism
Influenza A virus - physiology
Influenza viruses
Internal Medicine
Laboratory Medicine
Lectins
Neuraminidase - metabolism
Pathology
Permeability
Trypsin
Virion - metabolism
Virion - physiology
Virology
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Summary:Vesicles on the virion surface, which continued the lipoprotein membrane but had no spikes of virus glycoproteins hemagglutinin (HA) and neuraminidase (NA), were detected. These vesicles and virus particles were 18±7 and 103±12 nm in diameter, respectively, and, as a rule, one vesicle was found per virion. The locus of the external protrusion in the virion presumably corresponded to the site of virus budding during assembly in infected cell free from HA and NA spikes outside and M1 matrix protein inside, but enriched with ionic channel protein M2. Particles with vesicles constituted ~3-10% of the virus population produced in MDCK-H culture and containing uncleaved HA0 hemagglutinin. The content of vesicular virions increased slightly after trypsin cleavage HA0→HA1+HA2 and reached 10-15%. Exposure of the virus in acid medium (pH 4.3) led to a drastic increase of vesicular virions – to 60-80% for HA0 and HA1+HA2 virus. This was paralleled by changes in contrast permeability (for phosphotungstic acid). HA0 virions remained contrast-impermeable, while HA1+HA2 particles let the contrast in through the vesicles detected on the surface and were rapidly destroyed after incubation in acid medium. Hence, cleavage of the surface glycoprotein HA0 into HA1 and HA2 stimulated the acid-dependent permeability of the lipid membrane and led to attenuation of the ribonucleoprotein and protein matrix M1 contacts inside the virion.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-015-2860-6