RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots

Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland’s medium...

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Bibliographic Details
Published in:Plant molecular biology 2016-04, Vol.90 (6), p.589-603
Main Authors: Pankievicz, V. C. S, Camilios-Neto, D, Bonato, P, Balsanelli, E, Tadra-Sfeir, M. Z, Faoro, H, Chubatsu, L. S, Donatti, L, Wajnberg, G, Passetti, F, Monteiro, R. A, Pedrosa, F. O, Souza, E. M
Format: Article
Language:English
Subjects:
ABC transporters
Adaptation, Physiological - genetics
adhesins
auxins
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biochemistry
Biomedical and Life Sciences
cell walls
Chemotactic Factors - genetics
endophytes
epiphytes
gene expression
Gene Expression Regulation, Bacterial
genes
granules
grasses
growth promotion
Herbaspirillum - cytology
Herbaspirillum - genetics
Herbaspirillum - physiology
Herbaspirillum seropedicae
hydroponics
Indoleacetic Acids - metabolism
Life Sciences
messenger RNA
metabolism
microscopy
Nitrogen
nitrogen fixation
Nitrogen Fixation - genetics
Plant biology
plant growth
Plant Growth Regulators - genetics
Plant Growth Regulators - metabolism
Plant Pathology
Plant Roots - microbiology
Plant Sciences
polyhydroxybutyrate
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
Rhizosphere
ribosomal RNA
roots
seedlings
Seedlings - microbiology
Sequence Analysis, RNA
Soil Microbiology
Soil microorganisms
Symbiosis
transcription (genetics)
Transcriptome
Triticum - microbiology
Triticum aestivum
Wheat
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Summary:Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland’s medium were inoculated with H. seropedicae and incubated for 3 days. Total mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of root attached and planktonic bacteria revealed extensive metabolic adaptations to the epiphytic life style. These adaptations include expression of specific adhesins and cell wall re-modeling to attach to the root. Additionally, the metabolism was adapted to the microxic environment and nitrogen-fixation genes were expressed. Polyhydroxybutyrate (PHB) synthesis was activated, and PHB granules were stored as observed by microscopy. Genes related to plant growth promotion, such as auxin production were expressed. Many ABC transporter genes were regulated in the bacteria attached to the roots. The results provide new insights into the adaptation of H. seropedicae to the interaction with the plant.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-016-0430-6