The role of in-vivo and in-vitro maturation time on ooplasmic dysmaturity

Abstract This study investigates whether the timing of in-vivo and in-vitro maturation influences ooplasmic dysmaturity. This is a retrospective comparison of intracytoplasmic sperm injection (ICSI) cycles (index cycles) complicated by complete fertilization failure (CFF) to cycles with successful f...

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Bibliographic Details
Published in:Reproductive biomedicine online 2016-04, Vol.32 (4), p.401-406
Main Authors: Pereira, Nigel, Neri, Queenie V, Lekovich, Jovana P, Palermo, Gianpiero D, Rosenwaks, Zev
Format: Article
Language:English
Subjects:
Chorionic Gonadotropin - administration & dosage
Chorionic Gonadotropin - pharmacology
Female
Fertilization
Fertilization failure
Humans
ICSI
In Vitro Oocyte Maturation Techniques
nuclear/cytoplasmic asynchrony
Obstetrics and Gynecology
oocyte maturity
Oocytes - growth & development
ooplasmic maturity
Ovulation Induction
Retrospective Studies
Sperm Injections, Intracytoplasmic
Time Factors
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Summary:Abstract This study investigates whether the timing of in-vivo and in-vitro maturation influences ooplasmic dysmaturity. This is a retrospective comparison of intracytoplasmic sperm injection (ICSI) cycles (index cycles) complicated by complete fertilization failure (CFF) to cycles with successful fertilization in the same patient. The cycle following the index cycle was modified intentionally to increase fertilization. The times between human chorionic gonadotrophin (HCG) trigger and oocyte retrieval, HCG trigger and removal of cumulus cells, and HCG trigger and sperm injection were recorded. Fifteen patients were included. Compared with successful fertilization cycles, index (CFF) cycles showed a shorter time interval between HCG trigger and oocyte retrieval (2029.0 ± 16 versus 2195.0 ± 10 min; P < 0.001), HCG trigger and removal of cumulus cells (2201.4 ± 15 versus 2309.0 ± 23 min; P < 0.001) and oocyte retrieval and removal of cumulus cells (114.0 ± 13 versus 171.8 ± 15 min; P < 0.001). The interval between HCG trigger and ICSI was comparable between groups. Findings reveal novel patterns in time intervals between HCG trigger, oocyte retrieval, removal of cumulus cells and ICSI. Thus, modulating time intervals between HCG trigger, oocyte retrieval, removal of cumulus cells and ICSI to grant fertilization seems feasible.
ISSN:1472-6483
1472-6491
DOI:10.1016/j.rbmo.2016.01.007