PLGA nanofibers improves the antitumoral effect of daunorubicin

[Display omitted] •PLGA nanofibers loaded with daunorubicin (PLGA-DNR) were prepared by electrospinning process.•Sixty-five percent of the DNR was released in an initial burst over 8h and by 1224h, eighty-five percent of the DNR had been released, modelled by zero order kinetics.•In vitro experiment...

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Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2015-12, Vol.136, p.248-255
Main Authors: Guimarães, Pedro P.G., Oliveira, Michele F., Gomes, Alinne D.M., Gontijo, Sávio M.L., Cortés, Maria E., Campos, Paula P., Viana, Celso T.R., Andrade, Silvia P., Sinisterra, Rubén D.
Format: Article
Language:English
Subjects:
Animals
Antibiotics, Antineoplastic - pharmacology
Biomedical materials
Cell Line
Cell Line, Tumor
Daunorubicin
Daunorubicin - pharmacology
Differential scanning calorimetry
Differential thermal analysis
Drug delivery system
Electrospinning
Humans
In vitro testing
In vivo testing
In vivo tests
Lactic Acid - chemistry
Male
Mice
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Nanofibers
PLGA nanofibers
Polyglycolic Acid - chemistry
Surgical implants
X-Ray Diffraction
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Summary:[Display omitted] •PLGA nanofibers loaded with daunorubicin (PLGA-DNR) were prepared by electrospinning process.•Sixty-five percent of the DNR was released in an initial burst over 8h and by 1224h, eighty-five percent of the DNR had been released, modelled by zero order kinetics.•In vitro experiments demonstrated that the PLGA-DNR nanofibers had a higher cytotoxic effect to A431 tumor cells than free DNR.•In vivo data showed that the nanofibers had an antiangiogenic effect, no inflammatory potential and decreased of the vascularization. The objective of this study was to evaluate the in vivo anti-inflammatory angiogenesis activity and in vitro cytotoxicity on normal and cancer cell models of a drug delivery system consisting of poly(lactic-co-glycolic acid) nanofibers loaded with daunorubicin (PLGA-DNR) that were fabricated using an electrospinning process. The PLGA-DNR nanofibers were also characterized by thermogravimetric analysis (TGA), differential thermal analysis (DTA) and differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM) and confocal fluorescence microscopy. In vitro release of DNR from the nanofibers and its corresponding mechanism were also evaluated. Sixty-five percent of the DNR was released in an initial burst over 8h, and by 1224h, eighty-five percent of the DNR had been released. The Higuchi model yielded the best fit to the DNR release profile over the first 8h, and the corresponding data from 24 to 1224h could be modeled using zero-order kinetics. The PLGA-DNR nanofibers exhibited a higher cytotoxicity to A431 cells than free DNR but a cytotoxicity similar to free DNR against fibroblast cells. A higher antiangiogenic effect of PLGA nanofibers was observed in the in vivo data when compared to free DNR, and no inflammatory potential was observed for the nanofibers.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2015.09.005