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Cryopreservation of sea perch ( Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization
With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch ( Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw sperma...
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| Published in: | Aquaculture 2004-11, Vol.241 (1), p.517-528 |
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| Main Authors: | , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c446t-f967ae7554dde65659ec5e29d8ed0ae2733844b8978df9b0f74bd326ef5147993 |
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| cites | cdi_FETCH-LOGICAL-c446t-f967ae7554dde65659ec5e29d8ed0ae2733844b8978df9b0f74bd326ef5147993 |
| container_end_page | 528 |
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| container_title | Aquaculture |
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| creator | Ji, X.S. Chen, S.L. Tian, Y.S. Yu, G.C. Sha, Z.X. Xu, M.Y. Zhang, S.C. |
| description | With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch (
Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. Post-thaw motility of frozen sperm obtained with extender modified plaice Ringer solution (MPRS) was higher than those achieved with extenders D-15 and modified Mounib's medium (MMM). With MPRS, the most effective cryoprotectant was determined to 10% dimethyl sulphoxide (DMSO). Post-thaw motility of frozen semen was not significantly reduced when the volume of diluted semen in the cryovial was increased from 0.5 to 1.0 ml (
p>0.05). When the sperm/egg ratios varied from 320,000:1 to 20,000:1, fertilization rates of frozen semen cryopreserved for 3 days or 1 year in liquid nitrogen were not significantly different from that of fresh sperm (
p>0.05). In fertilization trials of 230-ml eggs with frozen semen cryopreserved for 3 days in liquid nitrogen, 84.8% fertilization rate and 70.1% hatching rate were obtained, which was similar to control (81.0±2.3% and 87.2±3.1%) (
p>0.05). Insemination of large egg batches (440-ml eggs) with frozen sperm cryopreserved for 1 year in liquid nitrogen resulted in high fertilization rates (83.5%) and hatching rates (90.0%) that resembled rates obtained with fresh (control) semen (
p>0.05). Scanning and transmission electron microscopic observation indicated that while most of frozen–thawed sperm remained morphologically normal, some exhibited more or less damage, which probably caused the decrease in motility and fertility of the post-thawed sperm. |
| doi_str_mv | 10.1016/j.aquaculture.2004.07.012 |
| format | article |
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Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. Post-thaw motility of frozen sperm obtained with extender modified plaice Ringer solution (MPRS) was higher than those achieved with extenders D-15 and modified Mounib's medium (MMM). With MPRS, the most effective cryoprotectant was determined to 10% dimethyl sulphoxide (DMSO). Post-thaw motility of frozen semen was not significantly reduced when the volume of diluted semen in the cryovial was increased from 0.5 to 1.0 ml (
p>0.05). When the sperm/egg ratios varied from 320,000:1 to 20,000:1, fertilization rates of frozen semen cryopreserved for 3 days or 1 year in liquid nitrogen were not significantly different from that of fresh sperm (
p>0.05). In fertilization trials of 230-ml eggs with frozen semen cryopreserved for 3 days in liquid nitrogen, 84.8% fertilization rate and 70.1% hatching rate were obtained, which was similar to control (81.0±2.3% and 87.2±3.1%) (
p>0.05). Insemination of large egg batches (440-ml eggs) with frozen sperm cryopreserved for 1 year in liquid nitrogen resulted in high fertilization rates (83.5%) and hatching rates (90.0%) that resembled rates obtained with fresh (control) semen (
p>0.05). Scanning and transmission electron microscopic observation indicated that while most of frozen–thawed sperm remained morphologically normal, some exhibited more or less damage, which probably caused the decrease in motility and fertility of the post-thawed sperm.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2004.07.012</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal aquaculture ; Animal productions ; Biological and medical sciences ; Cryopreservation ; Fertilization ; Fundamental and applied biological sciences. Psychology ; General aspects ; Lateolabrax japonicus ; Marine ; Motility score ; Sea perch ; Sperm</subject><ispartof>Aquaculture, 2004-11, Vol.241 (1), p.517-528</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-f967ae7554dde65659ec5e29d8ed0ae2733844b8978df9b0f74bd326ef5147993</citedby><cites>FETCH-LOGICAL-c446t-f967ae7554dde65659ec5e29d8ed0ae2733844b8978df9b0f74bd326ef5147993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27938,27939</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16399192$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Ji, X.S.</creatorcontrib><creatorcontrib>Chen, S.L.</creatorcontrib><creatorcontrib>Tian, Y.S.</creatorcontrib><creatorcontrib>Yu, G.C.</creatorcontrib><creatorcontrib>Sha, Z.X.</creatorcontrib><creatorcontrib>Xu, M.Y.</creatorcontrib><creatorcontrib>Zhang, S.C.</creatorcontrib><title>Cryopreservation of sea perch ( Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization</title><title>Aquaculture</title><description>With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch (
Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. Post-thaw motility of frozen sperm obtained with extender modified plaice Ringer solution (MPRS) was higher than those achieved with extenders D-15 and modified Mounib's medium (MMM). With MPRS, the most effective cryoprotectant was determined to 10% dimethyl sulphoxide (DMSO). Post-thaw motility of frozen semen was not significantly reduced when the volume of diluted semen in the cryovial was increased from 0.5 to 1.0 ml (
p>0.05). When the sperm/egg ratios varied from 320,000:1 to 20,000:1, fertilization rates of frozen semen cryopreserved for 3 days or 1 year in liquid nitrogen were not significantly different from that of fresh sperm (
p>0.05). In fertilization trials of 230-ml eggs with frozen semen cryopreserved for 3 days in liquid nitrogen, 84.8% fertilization rate and 70.1% hatching rate were obtained, which was similar to control (81.0±2.3% and 87.2±3.1%) (
p>0.05). Insemination of large egg batches (440-ml eggs) with frozen sperm cryopreserved for 1 year in liquid nitrogen resulted in high fertilization rates (83.5%) and hatching rates (90.0%) that resembled rates obtained with fresh (control) semen (
p>0.05). Scanning and transmission electron microscopic observation indicated that while most of frozen–thawed sperm remained morphologically normal, some exhibited more or less damage, which probably caused the decrease in motility and fertility of the post-thawed sperm.</description><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Biological and medical sciences</subject><subject>Cryopreservation</subject><subject>Fertilization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Lateolabrax japonicus</subject><subject>Marine</subject><subject>Motility score</subject><subject>Sea perch</subject><subject>Sperm</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqNkM2O1DAQhC0EEsPCO5gDCA4JduLY8RGN-JNG4gJnq2O3hUeZOGs7K2afHs_OSnDk1If6uqq7CHnNWcsZlx-OLdxuYLe5bAnbjjHRMtUy3j0hOz6qvhlk1z0luyqIZhSjfE5e5HxkjEk58B3Z9ukc14QZ0x2UEBcaPc0IdMVkf9F39AAF4wxTgt_0CGtcgt3ye5qrfoIS7yNQWBz1CDlMYQ7lTH1MdE3RbfZi2GQLM1YglSrfP4S8JM88zBlfPc4b8vPzpx_7r83h-5dv-4-HxgohS-O1VIBqGIRzKAc5aLQDdtqN6Bhgp_p-FGIatRqd1xPzSkyu7yT6gQuldX9D3l596zm3G-ZiTiFbnGdYMG7ZcKXUyHRXQX0FbYo5J_RmTeEE6Ww4M5eizdH8U7S5FG2YMrXouvvmMQQur_oEiw35r4HsteYPGfsrh_Xju4DJZBtwsehCQluMi-E_0v4A_hudgA</recordid><startdate>20041126</startdate><enddate>20041126</enddate><creator>Ji, X.S.</creator><creator>Chen, S.L.</creator><creator>Tian, Y.S.</creator><creator>Yu, G.C.</creator><creator>Sha, Z.X.</creator><creator>Xu, M.Y.</creator><creator>Zhang, S.C.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20041126</creationdate><title>Cryopreservation of sea perch ( Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization</title><author>Ji, X.S. ; Chen, S.L. ; Tian, Y.S. ; Yu, G.C. ; Sha, Z.X. ; Xu, M.Y. ; Zhang, S.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-f967ae7554dde65659ec5e29d8ed0ae2733844b8978df9b0f74bd326ef5147993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>Biological and medical sciences</topic><topic>Cryopreservation</topic><topic>Fertilization</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Lateolabrax japonicus</topic><topic>Marine</topic><topic>Motility score</topic><topic>Sea perch</topic><topic>Sperm</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ji, X.S.</creatorcontrib><creatorcontrib>Chen, S.L.</creatorcontrib><creatorcontrib>Tian, Y.S.</creatorcontrib><creatorcontrib>Yu, G.C.</creatorcontrib><creatorcontrib>Sha, Z.X.</creatorcontrib><creatorcontrib>Xu, M.Y.</creatorcontrib><creatorcontrib>Zhang, S.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ji, X.S.</au><au>Chen, S.L.</au><au>Tian, Y.S.</au><au>Yu, G.C.</au><au>Sha, Z.X.</au><au>Xu, M.Y.</au><au>Zhang, S.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of sea perch ( Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization</atitle><jtitle>Aquaculture</jtitle><date>2004-11-26</date><risdate>2004</risdate><volume>241</volume><issue>1</issue><spage>517</spage><epage>528</epage><pages>517-528</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch (
Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. Post-thaw motility of frozen sperm obtained with extender modified plaice Ringer solution (MPRS) was higher than those achieved with extenders D-15 and modified Mounib's medium (MMM). With MPRS, the most effective cryoprotectant was determined to 10% dimethyl sulphoxide (DMSO). Post-thaw motility of frozen semen was not significantly reduced when the volume of diluted semen in the cryovial was increased from 0.5 to 1.0 ml (
p>0.05). When the sperm/egg ratios varied from 320,000:1 to 20,000:1, fertilization rates of frozen semen cryopreserved for 3 days or 1 year in liquid nitrogen were not significantly different from that of fresh sperm (
p>0.05). In fertilization trials of 230-ml eggs with frozen semen cryopreserved for 3 days in liquid nitrogen, 84.8% fertilization rate and 70.1% hatching rate were obtained, which was similar to control (81.0±2.3% and 87.2±3.1%) (
p>0.05). Insemination of large egg batches (440-ml eggs) with frozen sperm cryopreserved for 1 year in liquid nitrogen resulted in high fertilization rates (83.5%) and hatching rates (90.0%) that resembled rates obtained with fresh (control) semen (
p>0.05). Scanning and transmission electron microscopic observation indicated that while most of frozen–thawed sperm remained morphologically normal, some exhibited more or less damage, which probably caused the decrease in motility and fertility of the post-thawed sperm.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2004.07.012</doi><tpages>12</tpages></addata></record> |
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| ispartof | Aquaculture, 2004-11, Vol.241 (1), p.517-528 |
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| source | ScienceDirect Freedom Collection |
| subjects | Animal aquaculture Animal productions Biological and medical sciences Cryopreservation Fertilization Fundamental and applied biological sciences. Psychology General aspects Lateolabrax japonicus Marine Motility score Sea perch Sperm |
| title | Cryopreservation of sea perch ( Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization |
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