Cryopreservation of sea perch ( Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization

With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch ( Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw sperma...

Full description

Saved in:
Bibliographic Details
Published in:Aquaculture 2004-11, Vol.241 (1), p.517-528
Main Authors: Ji, X.S., Chen, S.L., Tian, Y.S., Yu, G.C., Sha, Z.X., Xu, M.Y., Zhang, S.C.
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Biological and medical sciences
Cryopreservation
Fertilization
Fundamental and applied biological sciences. Psychology
General aspects
Lateolabrax japonicus
Marine
Motility score
Sea perch
Sperm
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch ( Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. Post-thaw motility of frozen sperm obtained with extender modified plaice Ringer solution (MPRS) was higher than those achieved with extenders D-15 and modified Mounib's medium (MMM). With MPRS, the most effective cryoprotectant was determined to 10% dimethyl sulphoxide (DMSO). Post-thaw motility of frozen semen was not significantly reduced when the volume of diluted semen in the cryovial was increased from 0.5 to 1.0 ml ( p>0.05). When the sperm/egg ratios varied from 320,000:1 to 20,000:1, fertilization rates of frozen semen cryopreserved for 3 days or 1 year in liquid nitrogen were not significantly different from that of fresh sperm ( p>0.05). In fertilization trials of 230-ml eggs with frozen semen cryopreserved for 3 days in liquid nitrogen, 84.8% fertilization rate and 70.1% hatching rate were obtained, which was similar to control (81.0±2.3% and 87.2±3.1%) ( p>0.05). Insemination of large egg batches (440-ml eggs) with frozen sperm cryopreserved for 1 year in liquid nitrogen resulted in high fertilization rates (83.5%) and hatching rates (90.0%) that resembled rates obtained with fresh (control) semen ( p>0.05). Scanning and transmission electron microscopic observation indicated that while most of frozen–thawed sperm remained morphologically normal, some exhibited more or less damage, which probably caused the decrease in motility and fertility of the post-thawed sperm.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2004.07.012