Temperature effect on proliferation and differentiation of satellite cells from turkeys with different growth rates

Poultry selected for growth have an inefficient thermoregulatory system and are more sensitive to temperature extremes. Satellite cells are precursors to skeletal muscle and mediate all posthatch muscle growth. Their physiological functions are affected by temperature. The objective of the current s...

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Bibliographic Details
Published in:Poultry science 2016-04, Vol.95 (4), p.934-947
Main Authors: Clark, D L, Coy, C S, Strasburg, G M, Reed, K M, Velleman, S G
Format: Article
Language:English
Subjects:
Acclimatization
Animals
Cell Differentiation
Cell Proliferation
Hot Temperature
Male
Pectoralis Muscles - growth & development
Pectoralis Muscles - physiology
Satellite Cells, Skeletal Muscle - physiology
Selection, Genetic
Turkeys - genetics
Turkeys - growth & development
Turkeys - physiology
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Summary:Poultry selected for growth have an inefficient thermoregulatory system and are more sensitive to temperature extremes. Satellite cells are precursors to skeletal muscle and mediate all posthatch muscle growth. Their physiological functions are affected by temperature. The objective of the current study was to determine how temperature affects satellite cells isolated from the pectoralis major (p. major) muscle (breast muscle) of turkeys selected for increased 16 wk body weight (F line) in comparison to a randombred control line (RBC2) from which the F line originated. Pectoralis major muscle satellite cells were thermally challenged by culturing between 33°C and 43°C to analyze the effects of cold and heat on proliferation and differentiation as compared to control temperature of 38°C. Expression levels of myogenic regulatory factors: myogenic differentiation factor 1 (MYOD1) and myogenin (MYOG) were quantified by quantitative polymerase chain reaction (qPCR). At all sampling times, proliferation increased at a linear rate across temperature in both the RBC2 and F lines. Differentiation also increased at a linear rate across temperature from 33 to 41°C at all sampling times in both the F and RBC2 lines. Satellite cells isolated from F line turkeys were more sensitive to both hot and cold temperatures as proliferation and differentiation increased to a greater extent across temperature (33 to 43°C) when compared with the RBC2 line. Expression of MYOD1 and MYOG increased as temperatures increased from 33 to 41°C at all sampling times in both the F and RBC2 lines. These results demonstrate that satellite cell function is sensitive to both cold and hot temperatures and p. major muscle satellite cells from F line turkeys are more sensitive to temperature extremes than RBC2 satellite cells.
ISSN:0032-5791
1525-3171
DOI:10.3382/ps/pev437