Structure and function of the archaeal exosome

The RNA‐degrading exosome in archaea is structurally very similar to the nine‐subunit core of the essential eukaryotic exosome and to bacterial polynucleotide phosphorylase (PNPase). In contrast to the eukaryotic exosome, PNPase and the archaeal exosome exhibit metal ion‐dependent, phosphorolytic ac...

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Published in:Wiley interdisciplinary reviews. RNA 2014-09, Vol.5 (5), p.623-635
Main Authors: Evguenieva-Hackenberg, Elena, Hou, Linlin, Glaeser, Stefanie, Klug, Gabriele
Format: Article
Language:English
Subjects:
Adenine
Adenosine - metabolism
Archaea
Archaeal Proteins - metabolism
Biodegradation
Catalytic Domain
Coevolution
Conflicts of interest
DNA Primase - genetics
DNA Primase - metabolism
Exosomes - genetics
Genome, Archaeal - genetics
Genomes
Halophilic archaea
Methanogenic bacteria
Phosphorylase
Poly(A)
Polymers - metabolism
Polynucleotide phosphorylase
Primase
Purines
Pyrimidines
Ribonuclease
RNA processing
RNA, Archaeal - biosynthesis
RNA-Binding Proteins - metabolism
Structure-function relationships
Sulfolobus solfataricus
Sulfolobus solfataricus - genetics
Surveillance
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Summary:The RNA‐degrading exosome in archaea is structurally very similar to the nine‐subunit core of the essential eukaryotic exosome and to bacterial polynucleotide phosphorylase (PNPase). In contrast to the eukaryotic exosome, PNPase and the archaeal exosome exhibit metal ion‐dependent, phosphorolytic activities and synthesize heteropolymeric RNA tails in addition to the exoribonucleolytic RNA degradation in 3′ → 5′ direction. The archaeal nine‐subunit exosome consists of four orthologs of eukaryotic exosomal subunits: the RNase PH‐domain‐containing subunits Rrp41 and Rrp42 form a hexameric ring with three active sites, whereas the S1‐domain‐containing subunits Rrp4 and Csl4 form an RNA‐binding trimeric cap on the top of the ring. In vivo, this cap contains Rrp4 and Csl4 in variable amounts. Rrp4 confers poly(A) specificity to the exosome, whereas Csl4 is involved in the interaction with the archaea‐specific subunit of the complex, the homolog of the bacterial primase DnaG. The archaeal DnaG is a highly conserved protein and its gene is present in all sequenced archaeal genomes, although the exosome was lost in halophilic archaea and some methanogens. In exosome‐containing archaea, DnaG is tightly associated with the exosome. It functions as an additional RNA‐binding subunit with poly(A) specificity in the reconstituted exosome of Sulfolobus solfataricus and enhances the degradation of adenine‐rich transcripts in vitro. Not only the RNA‐binding cap but also the hexameric Rrp41–Rrp42 ring alone shows substrate selectivity and prefers purines over pyrimidines. This implies a coevolution of the exosome and its RNA substrates resulting in 3′‐ends with different affinities to the exosome. WIREs RNA 2014, 5:623–635. doi: 10.1002/wrna.1234 This article is categorized under: RNA Interactions with Proteins and Other Molecules > Protein–RNA Interactions: Functional Implications RNA Processing > 3' End Processing RNA Turnover and Surveillance > Turnover/Surveillance Mechanisms
ISSN:1757-7004
1757-7012
DOI:10.1002/wrna.1234