Plasmids Encoding Foot-and-Mouth Disease Virus VP1 Epitopes Elicited Immune Responses in Mice and Swine and Protected Swine against Viral Infection

VP1 is a capsid protein of foot-and-mouth disease virus (FMDV) and contains epitopes of the virus. Plasmids encoding two VP1 epitopes (amino acid residues 141–160 and 200–213) and a host–self immunoglobulin molecule were constructed to produce a new type of FMD DNA vaccine. Two plasmids, namely, pCE...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2000-12, Vol.278 (1), p.27-35
Main Authors: Wong, H.T., Cheng, S.C.S., Chan, E.W.C., Sheng, Z.T., Yan, W.Y., Zheng, Z.X., Xie, Y.
Format: Article
Language:English
Subjects:
Animals
Animals, Suckling
Antibodies, Viral - blood
Aphthovirus - genetics
Aphthovirus - immunology
Capsid - immunology
Capsid - pharmacology
DNA vaccine
Epitopes - immunology
foot-and-mouth disease
Foot-and-Mouth Disease - immunology
Foot-and-Mouth Disease - prevention & control
foot-and-mouth disease virus
IgG carrier
Lethal Dose 50
Lymphocyte Activation
Mice
Neutralization Tests
pigs
plasmid pCEIM
Plasmids - administration & dosage
Plasmids - immunology
Swine
Swine Diseases - immunology
Swine Diseases - prevention & control
T-Lymphocytes - immunology
Vaccination
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Viral Vaccines - administration & dosage
VP1 epitopes
VP1 protein
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Summary:VP1 is a capsid protein of foot-and-mouth disease virus (FMDV) and contains epitopes of the virus. Plasmids encoding two VP1 epitopes (amino acid residues 141–160 and 200–213) and a host–self immunoglobulin molecule were constructed to produce a new type of FMD DNA vaccine. Two plasmids, namely, pCEIM and pCEIS, containing mouse immunoglobulin (IgG) or swine IgG were subjected to immunogenicity testing in mice and swine, respectively. In mice administrated pCEIM in the abdomen using a genegun, both FMDV-specific T-cell proliferation and neutralizing antibodies were detected. In swine immunized with pCEIS at the back of the ear, immune responses were achieved after the second administration. Swine showed a T-cell proliferative response with a stimulation index (SI) of up to 8.1 and a neutralizing antibody response that was able to protect suckling mice from 102 LD50 (lethal dose 50) FMDV challenge. To compare the immunogenicity of the DNA-based vaccine candidate, versus the protein-based vaccine candidates, a second group of swine was immunized with the protein F1-scIgG, which was encoded by the plasmid pCEIS. Injection with F1-scIgG elicited a T-cell proliferative response of SI < 1.7 and a neutralizing antibody response that protected suckling mice from up to 105 LD50 FMDV challenge. In the challenge test, three of three swine immunized with pCEIS were fully protected from FMDV challenge.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2000.0607