Molecular and histological responses in rat skin exposed to m-xylene

Solvents, surfactants, cutting fluids, hydrocarbons, and oils cause skin irritation by incompletely understood mechanisms. This study examined histological and molecular changes in rodent skin caused by brief topical exposures to m‐xylene. At 0, 1, 2, 4, and 6 h after 1‐h exposure, skin samples were...

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Published in:Journal of biochemical and molecular toxicology 2003, Vol.17 (2), p.92-94
Main Authors: Gunasekar, Palur G., Rogers, James V., Kabbur, Mahendra B., Garrett, Carol M., Brinkley, William W., McDougal, James N.
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
DNA - metabolism
Inducible Nitric Oxide Synthase
Interleukin-1 - biosynthesis
Interleukin-1α
Irritants - toxicity
m-Xylene
Male
Nitric Oxide Synthase - biosynthesis
Nitric Oxide Synthase Type II
Rats
Rats, Inbred F344
Skin - metabolism
Skin - pathology
Skin Irritation
Xylenes - toxicity
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Summary:Solvents, surfactants, cutting fluids, hydrocarbons, and oils cause skin irritation by incompletely understood mechanisms. This study examined histological and molecular changes in rodent skin caused by brief topical exposures to m‐xylene. At 0, 1, 2, 4, and 6 h after 1‐h exposure, skin samples were removed and analyzed for histopathological changes and interleukin‐1α (IL‐1α) and inducible nitric oxide synthase (iNOS) protein levels. Histopathological changes (epidermal–dermal separation and granulocyte infiltration) and increases in IL‐1α and iNOS protein expression occurred during our observation period. IL‐1α levels increased by 80% immediately after exposure and iNOS levels increased about 60% 4 hours after exposure. Our study demonstrates that dermal exposure to m‐xylene promotes IL‐1α and iNOS production in skin and these proteins may serve as early indicators of skin irritation. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:92–94, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10065
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.10065