Testing monophyly of the freshwater fish Leporinus (Characiformes, Anostomidae) through molecular analysis

Monophyly of the genus Leporinus (Characiformes: Anostomidae) was tested by sequencing and analysing a total of 4732 bp, including two mitochondrial [cytochrome oxidase subunit 1 (CO1) and cytochrome b (Cytb)] and three nuclear [myosin heavy chain 6 cardiac muscle alpha (Myh6), recombination activat...

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Bibliographic Details
Published in:Journal of fish biology 2016-03, Vol.88 (3), p.1204-1214
Main Authors: Ramirez, J. L., Carvalho-Costa, L. F., Venere, P. C., Carvalho, D. C., Troy, W. P., Galetti Jr, P. M.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Bayes Theorem
Bayesian species tree
Characiformes - classification
Characiformes - genetics
Cytochrome
Cytochromes b - genetics
DNA - chemistry
DNA - isolation & purification
DNA-Binding Proteins - genetics
Electron Transport Complex IV - genetics
Fresh Water
Genes, Mitochondrial
Genetic Markers
Homeodomain Proteins - genetics
Likelihood Functions
molecular systematics
Myosin Heavy Chains - genetics
paraphyly
Phylogeny
Sequence Alignment - veterinary
Sequence Analysis, DNA - veterinary
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Summary:Monophyly of the genus Leporinus (Characiformes: Anostomidae) was tested by sequencing and analysing a total of 4732 bp, including two mitochondrial [cytochrome oxidase subunit 1 (CO1) and cytochrome b (Cytb)] and three nuclear [myosin heavy chain 6 cardiac muscle alpha (Myh6), recombination activating gene 1 (RAG1) and recombination activating gene 2 (RAG2)] loci for 22 species of Leporinus, or c. 25% of all described species in the genus. Phylogenetic tree analyses (maximum parsimony, maximum likelihood and Bayesian species tree) indicate Leporinus to be paraphyletic, with monophyly being rejected by both Kishino–Hasegawa and Shimodaira–Hasegawa tests. The sequenced species of Leporinus are distributed across five clades that are interleaved among other anostomid genera. Several taxonomic changes are suggested as being necessary to restore monophyly for the group. The clade containing the type species, Leporinus fasciatus, should be considered Leporinus sensu stricto and at least three new genera should be described for other species currently considered part of Leporinus.
ISSN:0022-1112
1095-8649
DOI:10.1111/jfb.12906