Sampling large geographic areas for rare species using environmental DNA: a study of bull trout Salvelinus confluentus occupancy in western Montana

This study tested the efficacy of environmental DNA (eDNA) sampling to delineate the distribution of bull trout Salvelinus confluentus in headwater streams in western Montana, U.S.A. Surveys proved fast, reliable and sensitive: 124 samples were collected across five basins by a single crew in c. 8 d...

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Bibliographic Details
Published in:Journal of fish biology 2016-03, Vol.88 (3), p.1215-1222
Main Authors: McKelvey, K. S., Young, M. K., Knotek, W. L., Carim, K. J., Wilcox, T. M., Padgett-Stewart, T. M., Schwartz, M. K.
Format: Article
Language:English
Subjects:
Animals
Deoxyribonucleic acid
distribution
DNA
DNA - analysis
eDNA
Endangered & extinct species
Endangered Species - legislation & jurisprudence
Environment
Environmental Monitoring - methods
genetics
Montana
occupancy
quantitative PCR
Real-Time Polymerase Chain Reaction - veterinary
Rivers
Species Specificity
Trout - genetics
Trout - physiology
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Summary:This study tested the efficacy of environmental DNA (eDNA) sampling to delineate the distribution of bull trout Salvelinus confluentus in headwater streams in western Montana, U.S.A. Surveys proved fast, reliable and sensitive: 124 samples were collected across five basins by a single crew in c. 8 days. Results were largely consistent with past electrofishing, but, in a basin where S. confluentus were known to be scarce, eDNA samples indicated that S. confluentus were more broadly distributed than previously thought.
ISSN:0022-1112
1095-8649
DOI:10.1111/jfb.12863