3D printed nanocomposite matrix for the study of breast cancer bone metastasis

Abstract Bone is one of the most common metastatic sites of breast cancer, but the underlying mechanisms remain unclear, in part due to an absence of advanced platforms for cancer culture and study that mimic the bone microenvironment. In the present study, we integrated a novel stereolithography-ba...

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Bibliographic Details
Published in:Nanomedicine 2016-01, Vol.12 (1), p.69-79
Main Authors: Zhu, Wei, MS, Holmes, Benjamin, MS, Glazer, Robert I., PhD, Zhang, Lijie Grace, PhD
Format: Article
Language:English
Subjects:
3D printing
Batch Cell Culture Techniques - methods
Biomimetic Materials - chemical synthesis
Bone cancer metastasis
Bone Matrix - chemistry
Bone Neoplasms - pathology
Bone Neoplasms - secondary
Breast cancer
Breast Neoplasms - pathology
Cell Line, Tumor
Humans
Internal Medicine
Materials Testing
Nanocomposites - chemistry
Nanocomposites - ultrastructure
Nanomaterial
Neoplasm Invasiveness
Particle Size
Printing, Three-Dimensional
Tumor Microenvironment
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Summary:Abstract Bone is one of the most common metastatic sites of breast cancer, but the underlying mechanisms remain unclear, in part due to an absence of advanced platforms for cancer culture and study that mimic the bone microenvironment. In the present study, we integrated a novel stereolithography-based 3D printer and a unique 3D printed nano-ink consisting of hydroxyapatite nanoparticles suspended in hydrogel to create a biomimetic bone-specific environment for evaluating breast cancer bone invasion. Breast cancer cells cultured in a geometrically optimized matrix exhibited spheroid morphology and migratory characteristics. Co-culture of tumor cells with bone marrow mesenchymal stem cells increased the formation of spheroid clusters. The 3D matrix also allowed for higher drug resistance of breast cancer cells than 2D culture. These results validate that our 3D bone matrix can mimic tumor bone microenvironments, suggesting that it can serve as a tool for studying metastasis and assessing drug sensitivity. From the Clinical Editor Cancer remains a major cause of mortality for patients in the clinical setting. For breast cancer, bone is one of the most common metastatic sites. In this intriguing article, the authors developed a bone-like environment using 3D printing technology to investigate the underlying biology of bone metastasis. Their results would also allow a new model for other researchers who work on cancer to use.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2015.09.010