Effects of 10-hydroxycamptothecin on intrinsic mitochondrial pathway in silkworm BmN-SWU1 cells

10-Hydroxycamptothecin (HCPT), a plant alkaloid isolated from Camptotheca acuminate, is known as a planted-derived insecticide, however, the specific mechanism in insect cells is still unclear. In this study, we treated the ovarian cell line of the silkworm, BmN-SWU1, with different HCPT doses for d...

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Bibliographic Details
Published in:Pesticide biochemistry and physiology 2016-02, Vol.127, p.15-20
Main Authors: Pan, Chun, Hu, Yan-Fen, Song, Juan, Yi, Hua-Shan, Wang, La, Yang, Yi-Ying, Wang, Yong-Ping, Zhang, Man, Pan, Min-Hui, Lu, Cheng
Format: Article
Language:English
Subjects:
Animals
Apoptosis
BmN-SWU1 cell line
Bombyx
Bombyx mori
Camptotheca
Camptothecin - analogs & derivatives
Camptothecin - pharmacology
Cell Line
Female
HCPT
Mitochondria
Mitochondria - drug effects
Ovary - cytology
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Summary:10-Hydroxycamptothecin (HCPT), a plant alkaloid isolated from Camptotheca acuminate, is known as a planted-derived insecticide, however, the specific mechanism in insect cells is still unclear. In this study, we treated the ovarian cell line of the silkworm, BmN-SWU1, with different HCPT doses for durations ranging from 0 to 72h. The apoptosis morphology was evident after 72h of incubation and included cell protuberance, concentrated cytoplasm and apoptotic bodies. We observed DNA fragmentation and cell apoptosis after HCPT treatment. The disruption of mitochondrial distribution, activation of the intracellular mitochondrial permeability transition pore, and release of cytochrome c during HCPT-induced apoptosis in dose and time-dependent manner indicate the involvement of mitochondria in BmN-SWU1 cells. Caspase-9 and -3 activities increased gradually with the duration of incubation time. In conclusion, HCPT has a significant effect to initiate the intrinsic mitochondrial pathway in silkworm cells, providing a theoretical basis for better application of plant-derived insecticide in pest control. [Display omitted] •HCPT inhibited cell viability and induced apoptosis in BmN-SWU1 cell lines.•Mitochondrial morphology changed and mitochondrial Δψm reduced in cells exposed to HCPT.•Mitochondrial release of cytochrome c was associated with the loss of mitochondrial Δψm in HCPT-induced BmN-SWU1 cells.•Loss of mitochondrial membrane integrity and release of cytochrome c were key in mediating cell apoptosis.•The activation of caspase-3 was determined by the formation of apoptosome in HCPT-treated BmN-SWU1 cells.
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2015.09.001