Complementary resistance genes in wheat selection ‘Avocet R’ confer resistance to stripe rust

KEY MESSAGE : Complementary genes for resistance to wheat stripe rust in an Avocet selection mapped to chromosome arms 3DL and 5BL. Susceptible Avocet selections lacked the 5BL gene due to a chromosomal deletion. This study reports the inheritance and genetic mapping of the YrA (temporary name of co...

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Bibliographic Details
Published in:Theoretical and applied genetics 2016-01, Vol.129 (1), p.65-76
Main Authors: Dracatos, Peter M., Zhang, Peng, Park, Robert F., McIntosh, Robert A., Wellings, Colin R.
Format: Article
Language:English
Subjects:
Agriculture
Basidiomycota
Biochemistry
Biomedical and Life Sciences
Biotechnology
Chromosome Mapping
Chromosomes
Crosses, Genetic
Cultivars
Disease resistance (Plants)
Disease Resistance - genetics
Diseases and pests
Gene expression
Genes
Genes, Dominant
Genes, Plant
Genetic aspects
Genetic Linkage
Genotype
Haploidy
Health aspects
In Situ Hybridization, Fluorescence
Inheritance Patterns
Life Sciences
Observations
Original Article
Pathogens
Phenotype
Plant Biochemistry
Plant Breeding/Biotechnology
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Genetics and Genomics
Plant-pathogen relationships
Prevention
Puccinia striiformis
Rusts (Fungi)
Triticum - genetics
Triticum - microbiology
Triticum aestivum
Virulence
Wheat
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Summary:KEY MESSAGE : Complementary genes for resistance to wheat stripe rust in an Avocet selection mapped to chromosome arms 3DL and 5BL. Susceptible Avocet selections lacked the 5BL gene due to a chromosomal deletion. This study reports the inheritance and genetic mapping of the YrA (temporary name of convenience to describe the specificity) seedling resistance to wheat stripe rust (caused by Puccinia striiformis f. sp. tritici; Pst) in a resistant selection of the Australian cv. Avocet [Avocet R (AvR)–AUS 90660]. Genetic analysis was performed on F₂ populations and F₃ generation families from crosses between wheats that carried and lacked the YrA resistance. Greenhouse seedling tests with two avirulent Pst pathotypes (104 E137 A− and 108 E141 A−) confirmed that the YrA resistance was inherited as two complementary dominant genes. Ninety-two doubled haploid (DH) lines from a cross between the Australian cv. Teal (Pst susceptible) and AvR were used for DArT-Seq genotypic analysis to map the seedling resistance. Marker-trait association analysis using 9035 DArT-Seq loci mapped the genes to the long arms of chromosomes 3D (3DL) and 5B (5BL), respectively. F₂ populations from crosses between susceptible DH lines that carried either the 3DL or 5BL marker genotypes confirmed the complementary gene model. Fluorescence in situ hybridization (FISH) analysis determined that Teal carries a reciprocal T5B–7B translocation. FISH analysis also identified a 5BL chromosomal deletion in Avocet S relative to AvR that further validated the complementary gene model and possibly explained the heterogeneity of closely related wheats carrying the YrA resistance. The individual loci of the complementary YrA resistance were designated Yr73 (3DL) and Yr74 (5BL). Candidate single gene reference stocks will be permanently accessioned following cytological analysis to avoid the T5B–7B translocation.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-015-2609-7