The effect of a high-roughage diet on the metabolism of aromatic compounds by rumen microbes: a metagenomic study using Mehsani buffalo (Bubalus bubalis)

In developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. It is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. A metagenomic analysis was therefore carried out...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2016-02, Vol.100 (3), p.1319-1331
Main Authors: Prajapati, Vimalkumar S., Purohit, Hemant J., Raje, Dhananjay V., Parmar, Nidhi, Patel, Anand B., Jones, Oliver A. H., Joshi, Chaitanya G.
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Animal Feed - analysis
Animals
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Biodiversity
Biomedical and Life Sciences
Biotechnology
Bubalus bubalis
Buffaloes - metabolism
Buffaloes - microbiology
Cattle
Developing countries
Diet
Dietary Fiber - metabolism
Farming
Feed science
Feeds
Firmicutes
Gastrointestinal Microbiome
Gene expression
Genetic aspects
Genetic engineering
Genetic testing
Genomes
Genomics
Health aspects
LDCs
Life Sciences
Lignin
Livestock
Livestock industry
Metabolism
Metagenomics
Methane
Microbial Genetics and Genomics
Microbiology
Microbiota
Observations
Phylogeny
Proteomics
Rumen - metabolism
Rumen - microbiology
Studies
Transcriptomics
Volatile Organic Compounds - metabolism
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Summary:In developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. It is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. A metagenomic analysis was therefore carried out to assess the microbial communities associated with the liquid and solid fractions of rumen biomaterial from domestic Mehsani buffalo ( Bubalus bubalis ) fed with varying proportions of roughage. The experimental design consisted of three feeding regimes (50, 75 and 100 % roughage) and two roughage types (green and dry). Genes associated with aromatic compound degradation were assessed via high-throughput DNA sequencing. A total of 3914.94 Mb data were generated from all treatment groups. Genes coding for functional responses associated with aromatic compound metabolism were more prevalent in the liquid fraction of rumen samples than solid fractions. Statistically significant differences ( p  
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-015-7239-0