Whey protein concentrate enhances intestinal integrity and influences transforming growth factor-β1 and mitogen-activated protein kinase signalling pathways in piglets after lipopolysaccharide challenge

Whey protein concentrate (WPC) has been reported to have protective effects on the intestinal barrier. However, the molecular mechanisms involved are not fully elucidated. Transforming growth factor-β1 (TGF-β1) is an important component in the WPC, but whether TGF-β1 plays a role in these processes...

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Bibliographic Details
Published in:British journal of nutrition 2016-03, Vol.115 (6), p.984-993
Main Authors: Xiao, Kan, Jiao, Lefei, Cao, Shuting, Song, Zehe, Hu, Caihong, Han, Xinyan
Format: Article
Language:English
Subjects:
Animals
Crosses, Genetic
Cytokines - genetics
Cytokines - metabolism
Dietary Supplements
Disease Models, Animal
Electric Impedance
Enterocolitis - metabolism
Enterocolitis - pathology
Enterocolitis - physiopathology
Enterocolitis - prevention & control
Intestinal Mucosa - immunology
Intestinal Mucosa - metabolism
Intestinal Mucosa - pathology
Intestinal Mucosa - physiopathology
Intestines - immunology
Intestines - metabolism
Intestines - pathology
Intestines - physiopathology
Lipopolysaccharides - toxicity
Male
MAP Kinase Signaling System
Orchiectomy - veterinary
Permeability
Random Allocation
Sus scrofa
Tight Junction Proteins - genetics
Tight Junction Proteins - metabolism
Transforming Growth Factor beta1 - analysis
Transforming Growth Factor beta1 - genetics
Transforming Growth Factor beta1 - metabolism
Transforming Growth Factor beta1 - therapeutic use
Weaning
Whey Proteins - chemistry
Whey Proteins - therapeutic use
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Summary:Whey protein concentrate (WPC) has been reported to have protective effects on the intestinal barrier. However, the molecular mechanisms involved are not fully elucidated. Transforming growth factor-β1 (TGF-β1) is an important component in the WPC, but whether TGF-β1 plays a role in these processes is not clear. The aim of this study was to investigate the protective effects of WPC on the intestinal epithelial barrier as well as whether TGF-β1 is involved in these protection processes in a piglet model after lipopolysaccharide (LPS) challenge. In total, eighteen weanling pigs were randomly allocated to one of the following three treatment groups: (1) non-challenged control and control diet; (2) LPS-challenged control and control diet; (3) LPS+5 %WPC diet. After 19 d of feeding with control or 5 %WPC diets, pigs were injected with LPS or saline. At 4 h after injection, pigs were killed to harvest jejunal samples. The results showed that WPC improved (P
ISSN:1475-2662
DOI:10.1017/S0007114515005085