Visualizing the molecular sociology at the HeLa cell nuclear periphery

The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo–electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed th...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2016-02, Vol.351 (6276), p.969-972
Main Authors: Mahamid, Julia, Pfeffer, Stefan, Schaffer, Miroslava, Villa, Elizabeth, Danev, Radostin, Cuellar, Luis Kuhn, Förster, Friedrich, Hyman, Anthony A., Plitzko, Jürgen M., Baumeister, Wolfgang
Format: Article
Language:English
Subjects:
Cells
Cryoelectron Microscopy - methods
Cytoskeleton - metabolism
Cytoskeleton - ultrastructure
Electron Microscope Tomography - methods
Eukaryotes
HeLa Cells
Humans
Molecular biology
Nuclear Lamina - ultrastructure
Nuclear Pore - ultrastructure
Nucleosomes - ultrastructure
Protein Biosynthesis
Ribosomes - classification
Ribosomes - ultrastructure
Scientific imaging
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Summary:The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo–electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure—the nuclear pore complex—revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aad8857