Classification of reproductive toxicants with diverse mechanisms in the embryonic stem cell test

The embryonic stem cell test (EST) is a promising system to detect embryotoxicity in vitro. Recent studies have pointed out some limitations of the EST and suggest that the applicability domain of the EST and its prediction model have to be better defined. Here, eight substances of known reproductiv...

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Bibliographic Details
Published in:	Journal of toxicological sciences 2015/12/01, Vol.40(6), pp.809-815
Main Authors:	Riebeling, Christian, Fischer, Kristin, Luch, Andreas, Seiler, Andrea E.M.
Format:	Article
Language:	eng
Subjects:	3T3 Cells Acetates - toxicity Acetoacetates - toxicity Aminobutyrates - toxicity Animals Applicability domain Benzhydryl Compounds - toxicity Benzimidazoles - toxicity Cadmium Chloride - toxicity Carbamates - toxicity Cells, Cultured Developmental toxicity Embryonic stem cell test Embryonic Stem Cells - drug effects Embryotoxicity In vitro Mice Oxazoles - toxicity Phenols - toxicity Prediction model Sodium Chloride - toxicity Toxicity Tests - methods
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Summary:	The embryonic stem cell test (EST) is a promising system to detect embryotoxicity in vitro. Recent studies have pointed out some limitations of the EST and suggest that the applicability domain of the EST and its prediction model have to be better defined. Here, eight substances of known reproductive toxicity were tested in the EST under blind conditions. We applied the prediction model to the data of the EST after classifying the substances according to the published criteria. In addition, a simplified classification of the EST results into two classes as an approach to hazard assessment was compared to the European Union Classification, Labelling and Packaging (CLP) Regulation labels of the substances. With one exception, substances that are labeled as reproductive toxicants according to the CLP Regulation were detected as embryotoxic in the EST while substances without label were found to be non-embryotoxic according to the EST.
ISSN:	0388-1350 1880-3989