δ-Opioid receptor (DOR) signaling and reactive oxygen species (ROS) mediate intermittent hypoxia induced protection of canine myocardium

Intermittent, normobaric hypoxia confers robust cardioprotection against ischemia-induced myocardial infarction and lethal ventricular arrhythmias. δ-Opioid receptor (DOR) signaling and reactive oxygen species (ROS) have been implicated in cardioprotective phenomena, but their roles in intermittent...

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Bibliographic Details
Published in:Basic research in cardiology 2016-03, Vol.111 (2), p.17-17, Article 17
Main Authors: Estrada, Juan A., Williams, Arthur G., Sun, Jie, Gonzalez, Leticia, Downey, H. Fred, Caffrey, James L., Mallet, Robert T.
Format: Article
Language:English
Subjects:
Acetylcysteine
Animals
Arrhythmias, Cardiac - metabolism
Arrhythmias, Cardiac - prevention & control
Cardiology
Dogs
Female
Hematocrit
Hypoxia - metabolism
Ischemic Preconditioning, Myocardial - methods
Male
Medicine
Medicine & Public Health
Myocardial Infarction - metabolism
Myocardial Infarction - pathology
Myocardial Infarction - prevention & control
Myocardium - pathology
Naltrexone - analogs & derivatives
Original Contribution
Reactive Oxygen Species - metabolism
Receptors, Opioid, delta - metabolism
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Summary:Intermittent, normobaric hypoxia confers robust cardioprotection against ischemia-induced myocardial infarction and lethal ventricular arrhythmias. δ-Opioid receptor (DOR) signaling and reactive oxygen species (ROS) have been implicated in cardioprotective phenomena, but their roles in intermittent hypoxia are unknown. This study examined the contributions of DOR and ROS in mediating intermittent hypoxia-induced cardioprotection. Mongrel dogs completed a 20 day program consisting of 5–8 daily, 5–10 min cycles of moderate, normobaric hypoxia (FIO 2 0.095–0.10), with intervening 4 min room air exposures. Subsets of dogs received the DOR antagonist naltrindole (200 μg/kg, sc) or antioxidant N -acetylcysteine (250 mg/kg, po) before each hypoxia session. Twenty-four hours after the last session, the left anterior descending coronary artery was occluded for 60 min and then reperfused for 5 h. Arrhythmias detected by electrocardiography were scored according to the Lambeth II conventions. Left ventricles were sectioned and stained with 2,3,5-triphenyl-tetrazolium-chloride, and infarct sizes were expressed as percentages of the area at risk (IS/AAR). Intermittent hypoxia sharply decreased IS/AAR from 41 ± 5 % ( n  = 12) to 1.8 ± 0.9 % ( n  = 9; P  
ISSN:0300-8428
1435-1803
DOI:10.1007/s00395-016-0538-5