Mapping strategy for resistance genes against Cladosporium fulvum on the short arm of chromosome 1 of tomato: Cf-ECP5 near the Hcr9 Milky Way cluster

In the past, numerous Lycopersicon accessions have been described that harbor resistance genes to Cladosporium fulvum (Cf genes). Several Cf genes have been isolated, like Cf-4, Cf-4A and Cf-9, which are present on the short arm of Chromosome 1, and Cf-2 and Cf-5, which reside on Chromosome 6. To id...

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Bibliographic Details
Published in:Theoretical and applied genetics 2000-09, Vol.101 (4), p.661-668
Main Authors: Haanstra, J.P.W, Meijer-Dekens, F, Lauge, R, Seetanah, D.C, Joosten, M.H.A.J, Wit, P.J.G.M. de, Lindhout, P
Format: Article
Language:English
Subjects:
Biological and medical sciences
CARTE GENETIQUE
Cf gene
Chromosome 1
Chromosomes
CLADOSPORIUM
Cladosporium fulvum
Classical genetics, quantitative genetics, hybrids
Cultivars
DISEASE RESISTANCE
FULVIA FULVA
Fundamental and applied biological sciences. Psychology
GENE
GENES
GENETIC MAPS
GENETICA
GENETICS
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE
Hcr9 gene
LYCOPERSICON
MAPAS GENETICOS
PLANT PATHOGENIC FUNGI
Plant sciences
Pteridophyta, spermatophyta
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
Vegetals
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Summary:In the past, numerous Lycopersicon accessions have been described that harbor resistance genes to Cladosporium fulvum (Cf genes). Several Cf genes have been isolated, like Cf-4, Cf-4A and Cf-9, which are present on the short arm of Chromosome 1, and Cf-2 and Cf-5, which reside on Chromosome 6. To identify Cf genes linked to the Hcr9 cluster "Milky Way" on the short arm of Chromosome 1, we test-crossed 66 resistant Lycopersicon accessions to the near-isogenic line Moneymaker-Cf4, and the F^sub 1^s were crossed to the susceptible tomato cultivar Moneymaker. Putative linkage between an unknown Cf gene and Cf-4 was concluded based on small-scale allelic tests from an under-representation of susceptible genotypes in the progenies of 24 plants after inoculation with race 0 of C. fulvum. In this way, of the 21 resistant lines tested, 10 harbored a Cf gene that was linked to the Hcr9 Milky Way cluster. Moreover, one of the lines harboring a Cf gene closely linked to Cf-4 specifically recognizes the extracellular protein ECP5 of C. fulvum and was designated Cf-ECP5. Using a testcross population of 338 plants, we mapped Cf-ECP5 more accurately at 4 cM proximal to the Hcr9 Milky Way locus. This report shows that the method of small-scale allelic tests provides a useful tool to rapidly screen for Cf genes on the short arm of Chromosome 1. Further analysis of these Cf genes will elucidate the complex genetic organization of Cf genes on Chromosome 1 of tomato.[PUBLICATION ABSTRACT]
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220051528