Thyroid Oxidase (THOX2) Gene Expression in the Rat Thyroid Cell Line FRTL-5

A cDNA encoding an NADPH oxidase flavoprotein was isolated from the rat thyroid gland. The predicted 1517-residue polypeptide was 82.5% identical to the human THOX2/DUOX2 and 74% similar to THOX1/DUOX1. Rat THOX2 lacks a stretch of 30 residues, corresponding to one exon in the human gene sequence. T...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-10, Vol.277 (2), p.287-292
Main Authors: Dupuy, Corinne, Pomerance, Martine, Ohayon, Renée, Noël-Hudson, Marie-Sophie, Dème, Danielle, Chaaraoui, Mokhtar, Francon, Jacques, Virion, Alain
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antithyroid Agents - pharmacology
Blotting, Northern
Carcinogens
Cell Line
Cloning, Molecular
Colforsin - pharmacology
Cyclic AMP - metabolism
DNA, Complementary - metabolism
Exons
Female
Flavoproteins - biosynthesis
Flavoproteins - genetics
Humans
Hydrogen Peroxide - pharmacology
Iodide Peroxidase - chemistry
Iodide Peroxidase - genetics
Methimazole - pharmacology
Molecular Sequence Data
NADPH oxidase
Peroxidase - chemistry
Peroxidase - genetics
Protein Structure, Tertiary
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tetradecanoylphorbol Acetate - pharmacology
THOX2 gene
Thyroid Gland - enzymology
Thyrotropin - pharmacology
Time Factors
Tissue Distribution
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Summary:A cDNA encoding an NADPH oxidase flavoprotein was isolated from the rat thyroid gland. The predicted 1517-residue polypeptide was 82.5% identical to the human THOX2/DUOX2 and 74% similar to THOX1/DUOX1. Rat THOX2 lacks a stretch of 30 residues, corresponding to one exon in the human gene sequence. THOX2 mRNA was found to be expressed in cultured FRTL-5 cells. The level of THOX2 mRNA was increased by cAMP in these cells and it was decreased in the thyroids of rats treated with the antithyroid drug methimazole, unlike the TPO and NIS mRNAs. Since it was found in the intestine, duodenum, and colon, in addition to thyroid, we suggest that it be called LNOX, the new family of long homologs of NOX flavoproteins rather than THOX and/or DUOX.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.3671