Implication of Akt, ERK1/2 and alternative p38MAPK signalling pathways in human colon cancer cell apoptosis induced by green tea EGCG

We investigated apoptosis induced by the green tea component the epigallocatechin-3-gallate (EGCG) and the pathways underlying its activity in a colon cancer cell line. A complete understanding of the mechanism(s) and molecules targeted by green tea polyphenols could be useful in developing novel th...

Full description

Saved in:
Bibliographic Details
Published in:Food and chemical toxicology 2015-10, Vol.84, p.125-132
Main Authors: Cerezo-Guisado, María Isabel, Zur, Rafal, Lorenzo, María Jesús, Risco, Ana, Martín-Serrano, Miguel A., Alvarez-Barrientos, Alberto, Cuenda, Ana, Centeno, Francisco
Format: Article
Language:English
Subjects:
Akt
Anticarcinogenic Agents - metabolism
Antioxidants - metabolism
Apoptosis
Apoptosis - drug effects
Catechin - analogs & derivatives
Catechin - metabolism
Cell Line, Tumor
Colon cancer
Colonic Neoplasms - chemically induced
Colonic Neoplasms - enzymology
Colonic Neoplasms - metabolism
Colonic Neoplasms - prevention & control
EGCG
ERK
Food Handling
HEK293 Cells
Humans
Isoenzymes - antagonists & inhibitors
Isoenzymes - chemistry
Isoenzymes - genetics
Isoenzymes - metabolism
MAP Kinase Signaling System - drug effects
Osmolar Concentration
p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors
p38 Mitogen-Activated Protein Kinases - chemistry
p38 Mitogen-Activated Protein Kinases - genetics
p38 Mitogen-Activated Protein Kinases - metabolism
p38γ/p38δ
Phosphorylation - drug effects
Protein Kinase Inhibitors - pharmacology
Protein Processing, Post-Translational - drug effects
Proto-Oncogene Proteins c-akt - agonists
Proto-Oncogene Proteins c-akt - antagonists & inhibitors
Proto-Oncogene Proteins c-akt - metabolism
RNA Interference
RNA, Small Interfering
Tea - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We investigated apoptosis induced by the green tea component the epigallocatechin-3-gallate (EGCG) and the pathways underlying its activity in a colon cancer cell line. A complete understanding of the mechanism(s) and molecules targeted by green tea polyphenols could be useful in developing novel therapeutic approaches for cancer treatment. EGCG, which is the major polyphenol in green tea, has cytotoxic effects and induced cell death in HT-29 cell death. In this study, we evaluated the effect EGCG on mitogen-activated protein kinase (MAPK) and Akt pathways. EGCG treatment increased phospho-ERK1/2, -JNK1/2 and -p38α, -p38γ and -p38δ, as well as phospho-Akt levels. Using a combination of kinase inhibitors, we found that EGCG-induced cell death is partially blocked by inhibiting Akt, ERK1/2 or alternative p38MAPK activity. Our data suggest that these kinase pathways are involved in the anti-cancer effects of EGCG and indicate potential use of this compound as chemotherapeutic agent for colon cancer treatment. •Evidence supports a preventive role for epigallocatechin-3-gallate (EGCG), the major polyphenol in green tea, in cancer.•EGCG-induced cell death on the HT-29 human colon adenocarcinoma cell line.•Akt, ERK1/2 and p38δ pathways are implicated in EGCG-induced cell death.
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2015.08.017