Quantitative Multicolor Subdiffraction Imaging of Bacterial Protein Ultrastructures in Three Dimensions

We demonstrate quantitative multicolor three-dimensional (3D) subdiffraction imaging of the structural arrangement of fluorescent protein fusions in living Caulobacter crescentus bacteria. Given single-molecule localization precisions of 20–40 nm, a flexible locally weighted image registration algor...

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Bibliographic Details
Published in:Nano letters 2013-03, Vol.13 (3), p.987-993
Main Authors: Gahlmann, Andreas, Ptacin, Jerod L, Grover, Ginni, Quirin, Sean, von Diezmann, Alexander R. S, Lee, Marissa K, Backlund, Mikael P, Shapiro, Lucy, Piestun, Rafael, Moerner, W. E
Format: Article
Language:English
Subjects:
Algorithms
Applied sciences
Bacteria
Bacterial Proteins - ultrastructure
Caulobacter crescentus
Caulobacter crescentus - chemistry
Color
Condensed matter: structure, mechanical and thermal properties
Dielectrics
Electronics
Exact sciences and technology
Fluorescence
Image registration
Imaging
Molecular electronics, nanoelectronics
Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals
Physics
Proteins
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
Structure of solids and liquids
crystallography
Three dimensional
Wavelengths
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Summary:We demonstrate quantitative multicolor three-dimensional (3D) subdiffraction imaging of the structural arrangement of fluorescent protein fusions in living Caulobacter crescentus bacteria. Given single-molecule localization precisions of 20–40 nm, a flexible locally weighted image registration algorithm is critical to accurately combine the super-resolution data with
ISSN:1530-6984
1530-6992
DOI:10.1021/nl304071h