Liver-cell patterning lab chip: mimicking the morphology of liver lobule tissue

A lobule-mimetic cell-patterning technique for on-chip reconstruction of centimetre-scale liver tissue of heterogeneous hepatic and endothelial cells via an enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported. By mimicking the basic morphology of liver tissue, the classi...

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Bibliographic Details
Published in:Lab on a chip 2013-09, Vol.13 (18), p.3578-3587
Main Authors: Ho, Chen-Ta, Lin, Ruei-Zeng, Chen, Rong-Jhe, Chin, Chung-Kuang, Gong, Song-En, Chang, Hwan-You, Peng, Hwei-Ling, Hsu, Long, Yew, Tri-Rung, Chang, Shau-Feng, Liu, Cheng-Hsien
Format: Article
Language:English
Subjects:
Alignment
Arrays
Biomimetic Materials - chemistry
Cell Survival
Chambers
Chips
Cytochrome P-450 CYP1A1 - metabolism
Electrodes
Endothelial cells
Hep G2 Cells
Human Umbilical Vein Endothelial Cells
Humans
Liver
Liver - cytology
Liver - metabolism
Microfluidic Analytical Techniques - instrumentation
Morphology
Patterning
Regenerative Medicine
Tissue Engineering
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Summary:A lobule-mimetic cell-patterning technique for on-chip reconstruction of centimetre-scale liver tissue of heterogeneous hepatic and endothelial cells via an enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported. By mimicking the basic morphology of liver tissue, the classic hepatic lobule, the lobule-mimetic-stellate-electrodes array was designed for cell patterning. Through DEP manipulation, well-defined and enhanced spatial electric field gradients were created for in-parallel manipulation of massive individual cells. With this liver-cell patterning labchip design, the original randomly distributed hepatic and endothelial cells inside the microfluidic chamber can be manipulated separately and aligned into the desired pattern that mimicks the morphology of liver lobule tissue. Experimental results showed that both hepatic and endothelial cells were orderly guided, snared, and aligned along the field-induced orientation to form the lobule-mimetic pattern. About 95% cell viability of hepatic and endothelial cells was also observed after cell-patterning demonstration via a fluorescent assay technique. The liver function of CYP450-1A1 enzyme activity showed an 80% enhancement for our engineered liver tissue (HepG2+HUVECs) compared to the non-patterned pure HepG2 for two-day culturing.
ISSN:1473-0197
1473-0189
DOI:10.1039/c3lc50402f