Study of embryo rescue in floribunda rose

In the past few decades, breeders have faced a lot of problems in rose improvement due to low sexual reproduction and poor germination because of embryo abortion. Immature embryos may be recovered in vitro. An efficient protocol for embryo rescue in two floribunda roses ('Arunima' and ...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2005-04, Vol.81 (1), p.113-117
Main Authors: Mohapatra, A, Rout, G.R
Format: Article
Language:English
Subjects:
2,4-D
benzyladenine
Biological and medical sciences
Biotechnology
culture media
dose response
embryo (plant)
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
gibberellic acid
in vitro culture
in vitro regeneration
indole acetic acid
kinetin
methodology
Methods. Procedures. Technologies
micropropagation
Miscellaneous
ornamental plants
Plant cells and fungal cells
Rosa
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Summary:In the past few decades, breeders have faced a lot of problems in rose improvement due to low sexual reproduction and poor germination because of embryo abortion. Immature embryos may be recovered in vitro. An efficient protocol for embryo rescue in two floribunda roses ('Arunima' and 'Shocking Blue') was developed. The germination of immature embryos was achieved by manipulating the growth media, growth hormones and culture conditions. The embryos (rescued) germinated and grew considerably on Murashige and Skoog basal medium supplemented with 2.5 mg l super(-l) BA (6-benzylaminopurine), 0.5 mg l super(-l) GA sub(3) (gibberellic acid) and 3 (w/v) sucrose under 16-h photoperiod. A higher rate of germination was observed in cultures incubated 2 weeks in dark and subsequently transferred to 2 weeks in light at 16-h photoperiod. The embryo derived plantlets were successfully transferred to greenhouse and produced flowers. Embryo rescue technique in floribunda roses has great potential in floriculture industry.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-004-3128-4