Comparison among different cryoprotectants for cryopreservation of epididymal sperm from agouti (Dasyprocta leporina)

We verify the effects of different cryoprotectants on the cryopreservation of agouti (Dasyprocta leporina) epididymal sperm. We used 16 pairs of testes–epididymis complexes of sexually mature animals. We immediately evaluated epididymal sperm obtained by retrograde flushing for concentration, motili...

Full description

Saved in:
Bibliographic Details
Published in:Cryobiology 2015-12, Vol.71 (3), p.442-447
Main Authors: Castelo, T.S., Silva, A.M., Bezerra, L.G.P., Costa, C.Y.M., Lago, A.E.A., Bezerra, J.A.B., Campos, L.B., Praxedes, E.C.G., Silva, A.R.
Format: Article
Language:English
Subjects:
Agouti epididymal sperm
Animals
Cell Survival - drug effects
Cryopreservation
Cryopreservation - methods
Cryoprotectant
Cryoprotective Agents - pharmacology
Dasyprocta aguti
Dasyproctidae
Dimethyl Sulfoxide - pharmacology
Dimethylformamide - pharmacology
Epididymis - drug effects
Ethylene Glycol - pharmacology
Freezing
Germplasm bank
Glycerol - pharmacology
Male
Semen Preservation - methods
Sperm Motility - drug effects
Spermatozoa - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We verify the effects of different cryoprotectants on the cryopreservation of agouti (Dasyprocta leporina) epididymal sperm. We used 16 pairs of testes–epididymis complexes of sexually mature animals. We immediately evaluated epididymal sperm obtained by retrograde flushing for concentration, motility, vigor, viability, osmotic response, and morphology. Samples were extended in a coconut water extender plus 20% egg yolk, containing glycerol, ethylene glycol, dimethylsulfoxide – DMSO, or dimethylformamide. Finally, samples were stored in 0.25 mL straws, frozen in liquid nitrogen, and thawed after one week, being reevaluated and assessed for membrane integrity using fluorescent probes. The higher values for postthawing sperm motility, vigor, and membrane integrity were achieved by the usage of glycerol, when compared to ethylene glycol and dimethylformamide (P  0.05). All cryoprotectants provided a similar effect on the preservation of sperm morphology, osmotic response, and viability (P > 0.05). Therefore, here onwards, there was testing of glycerol and DMSO at 3 and 6% concentrations using the same freezing–thawing protocol reported previously. As the main result, DMSO at 6% concentration provided a decrease in sperm parameters, as well as in the chromatin integrity and in the binding capability of sperm. In conclusion, glycerol 3 or 6% and DMSO 3% can be used as alternative cryoprotectants for agouti epididymal sperm cryopreservation.
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2015.09.005