Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum

Ethylene responsive factors (ERFs) are important in regulating plant pathogen resistance, abiotic stress tolerance and plant development. Recent studies have greatly enlarged the ERF protein family and revealed more important roles of ERFs in plants. Here, we report our finding of a tomato ERF prote...

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Bibliographic Details
Published in:Plant molecular biology 2004-08, Vol.55 (6), p.825-834
Main Authors: Zhang, H, Zhang, D, Chen, J, Yang, Y, Huang, Z, Huang, D, Wang, X.C, Huang, R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
bacterial wilt
Base Sequence
beta-glucuronidase
binding capacity
Binding Sites - genetics
Blotting, Northern
disease resistance
DNA, Complementary - chemistry
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Electrophoretic Mobility Shift Assay
ethylene
ethylene responsive element
ethylene responsive factors
Ethylenes - pharmacology
gene expression
Gene Expression Regulation, Plant - drug effects
gene induction
gene up-regulation
Immunity, Innate
Lycopersicon esculentum
Molecular Sequence Data
Mutation
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana - microbiology
Nicotiana tabacum
Oligonucleotides - genetics
Oligonucleotides - metabolism
pathogenesis-related proteins
Plant Diseases - genetics
Plant Diseases - microbiology
plant pathogenic bacteria
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Genetically Modified
promoter regions
Protein Binding
Ralstonia solanacearum
Ralstonia solanacearum - growth & development
reporter genes
Response Elements - genetics
RNA, Plant - genetics
RNA, Plant - metabolism
Salicylic Acid - pharmacology
Sequence Analysis, DNA
Sequence Homology, Amino Acid
signal transduction
Solanum lycopersicum - genetics
Solanum lycopersicum - metabolism
Solanum lycopersicum - microbiology
Solanum lycopersicum var. lycopersicum
tobacco
tomatoes
Trans-Activators - genetics
Trans-Activators - metabolism
transcription factors
transgenic plants
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Summary:Ethylene responsive factors (ERFs) are important in regulating plant pathogen resistance, abiotic stress tolerance and plant development. Recent studies have greatly enlarged the ERF protein family and revealed more important roles of ERFs in plants. Here, we report our finding of a tomato ERF protein TSRF1, which is transcriptionally up-regulated by ethylene, salicylic acid, or Ralstonia solanacearum strain BJ1057 infection. Biochemical analysis indicates that TSRF1 specifically interacts in vitro with the GCC box, an element present in the promoters of many pathogenesis-related (PR) genes. Further investigation evidences that TSRF1 activates in vivo the expression of reporter beta-glucuronidase gene controlled by GCC box. More importantly, overexpressing TSRF1 in tobacco and tomato constitutively activates the expression of PR genes, and subsequently enhancing transgenic plant resistance to the bacterial wilt caused by Ralstonia solanacearum strain BJ1057. Therefore our investigation not only extends the functions of ERF proteins in plant resistance to R. solanacearum, but also provides further clues to understanding the mechanism of host regulatory proteins in response to the infection of pathogens.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-005-2140-3