Molecular investigations of Hepatozoon species in dogs and developmental stages of Rhipicephalus sanguineus

The occurrence and distribution of Hepatozoon species in stray dogs, and the developmental stages of Rhipicephalus sanguineus detached from the same dogs in Diyarbakır Province, Turkey is reported. A total of 328 ticks, including 133 adults (55 males and 75 females consist of 63 partially engorged a...

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Bibliographic Details
Published in:Parasitology research (1987) 2013-06, Vol.112 (6), p.2381-2385
Main Authors: Aktas, Munir, Özübek, Sezayi, Ipek, Duygu Neval Sayın
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Canis
Coccidia - classification
Coccidia - genetics
Coccidia - isolation & purification
Coccidiosis - complications
Coccidiosis - parasitology
Coccidiosis - veterinary
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
Dog Diseases - parasitology
Dogs
Ectoparasitic Infestations - complications
Ectoparasitic Infestations - parasitology
Ectoparasitic Infestations - veterinary
Felis
Genotype
Health aspects
Hepatozoon
Hepatozoon canis
Host-parasite relationships
Immunology
Ixodidae
Medical Microbiology
Microbiology
Molecular biology
Molecular Sequence Data
Original Paper
Physiological aspects
Polymerase Chain Reaction
Rhipicephalus sanguineus
Rhipicephalus sanguineus - growth & development
Sequence Analysis, DNA
Sporozoa
Ticks
Turkey
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Summary:The occurrence and distribution of Hepatozoon species in stray dogs, and the developmental stages of Rhipicephalus sanguineus detached from the same dogs in Diyarbakır Province, Turkey is reported. A total of 328 ticks, including 133 adults (55 males and 75 females consist of 63 partially engorged and 15 fully engorged) and 195 nymphs (91 partially engorged and 104 fully engorged) were detached from the dogs. Fully engorged nymphs and females were incubated at 27 °C and relative humidity of 85 % to molt to adult stage and recover eggs. The ticks were pooled according to sex and developmental stage. No Hepatozoon gamonts were found, whereas, by PCR, 15.87 % (10/63) of the dogs were infected with Hepatozoon canis . Of the 68 tick pools tested, 14 (20.58 %) pools were infected with Hepatozoon spp., an overall maximum likelihood estimation of prevalence of 4.9 % (95 % confidence intervals (CI) = 2.85–7.93 %) per 100 ticks. Maximum likelihood estimation of the infection rate varied by tick sex and developmental categories, ranging from 1.75 % (95 % CI = 0.11–8.11 %) in fed males to 6.81 % (95 % CI = 2.07–17.46 %) in unfed females. One amplicon from a fed adult female was 99 % identical to the sequence for Hepatozoon felis . The remaining sequences isolated from both dogs and ticks shared 99–100 % similarity with the corresponding H . canis isolates. This is the first detection of H. canis and H. felis in the tick R. sanguineus in Turkey.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-013-3403-6