Improvement of chlorophyll identification in foodstuffs by MALDI ToF/ToF mass spectrometry using 1,5-diaminonaphthalene electron transfer secondary reaction matrix

Chlorophylls (Chls) are important pigments responsible for the characteristic green color of chloroplasts in algae and plants. In this study, 1,5-diaminonaphthalene (DAN) was introduced as an electron transfer secondary reaction matrix for the identification of intact chlorophylls and their derivati...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2015-08, Vol.407 (21), p.6369-6379
Main Authors: Calvano, Cosima Damiana, Ventura, Giovanni, Cataldi, Tommaso R. I, Palmisano, Francesco
Format: Article
Language:English
Subjects:
2-Naphthylamine - analogs & derivatives
2-Naphthylamine - chemistry
Acids
Algae
Analytical Chemistry
Biochemistry
biotransformation
Byproducts
cations
Characterization and Evaluation of Materials
Chemical properties
Chemical reactions
Chemistry
Chemistry and Materials Science
Chlorophyll
Chlorophyll - analysis
Chlorophylls
chloroplasts
Chromatography
color
desorption
Electron transfer
Electron transport
Electrons
Food Analysis - methods
Food Science
Food testing
foods
Fragmentation
High-Resolution Mass Spectrometry in Food and Environmental Analysis
Identification and classification
Ionization
Laboratory Medicine
Lasers
leaves
Mass spectrometry
Methods
Monitoring/Environmental Analysis
Observations
Pigments
Plant Leaves - chemistry
Plant Oils - analysis
Research Paper
Scientific imaging
Seeds
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Sunflower Oil
tea
Tea - chemistry
Time-of-flight mass spectrometry
vegetable oil
Vegetables
Vegetables - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chlorophylls (Chls) are important pigments responsible for the characteristic green color of chloroplasts in algae and plants. In this study, 1,5-diaminonaphthalene (DAN) was introduced as an electron transfer secondary reaction matrix for the identification of intact chlorophylls and their derivatives, by matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS). DAN was proved to drastically outperform conventional matrices such as α-cyano-4-hydroxycinnnamic acid, dithranol, antracene, and even terthiophene, since loss of the metal ion and fragmentation of the phytol–ester linkage are negligible. Absence of significant fragmentation of radical cations of Chls a and b at m/z 892.529 and 906.513, respectively, makes MALDI MS capable of following natural degradation of intact porphyrin-based pigments whose initial steps are just represented by demetalation and dephytylation. Chl by-products, such as pyropheophytins, have been identified in dried tea leaves showing the potential of MALDI MS to follow chlorophyll biotransformation occurring in processed foodstuffs. Finally, preliminary results show the potential of MALDI MS to detect illegal vegetable oil re-greening practices.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-015-8728-9