Modulation by gamithromycin and ketoprofen of in vitro and in vivo porcine lipopolysaccharide-induced inflammation

The immunomodulatory properties of gamithromycin (GAM), ketoprofen (KETO) and their combination (GAM-KETO) were investigated after both in vitro and in vivo lipopolysaccharide (LPS)-induced inflammation. The influence of these drugs was measured on the production of prostaglandin E2 (PGE2) and the p...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2015-12, Vol.168 (3-4), p.211-222
Main Authors: Wyns, Heidi, Meyer, Evelyne, Plessers, Elke, Watteyn, Anneleen, van Bergen, Thomas, Schauvliege, Stijn, De Baere, Siegrid, Devreese, Mathias, De Backer, Patrick, Croubels, Siska
Format: Article
Language:English
Subjects:
Acute-Phase Proteins - genetics
Acute-Phase Proteins - metabolism
Animals
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - therapeutic use
Anti-Inflammatory Agents, Non-Steroidal - administration & dosage
Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
Cells, Cultured
Cytokines - genetics
Cytokines - metabolism
Dinoprostone - genetics
Dinoprostone - metabolism
Drug Therapy, Combination
Gamithromycin
Gene Expression Regulation - drug effects
Inflammation - chemically induced
Inflammation - drug therapy
Interleukin-1β
Interleukin-6
Ketoprofen
Ketoprofen - administration & dosage
Ketoprofen - therapeutic use
Leukocytes, Mononuclear - drug effects
Lipopolysaccharide
Lipopolysaccharides - toxicity
Macrolides - administration & dosage
Macrolides - therapeutic use
Male
PBMC
Pig
Prostaglandin
Swine
Swine Diseases - chemically induced
Swine Diseases - drug therapy
Tumour necrosis factor-α
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Summary:The immunomodulatory properties of gamithromycin (GAM), ketoprofen (KETO) and their combination (GAM-KETO) were investigated after both in vitro and in vivo lipopolysaccharide (LPS)-induced inflammation. The influence of these drugs was measured on the production of prostaglandin E2 (PGE2) and the pro-inflammatory cytokines tumour necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β in both LPS-stimulated porcine peripheral blood mononuclear cells (PBMCs) and LPS-challenged pigs. Additionally, effects on the production of acute phase proteins (APPs), including pig major acute phase protein (pig-MAP) and C-reactive protein (CRP), as well as on the development of fever, pulmonary symptoms and sickness behaviour were investigated. Dexamethasone was included as a positive control in the in vitro research. Following an 18h-incubation period with 1.25μg/mL LPS, the levels of TNF-α, IL-1β and IL-6 (p
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2015.09.014