Kinase Gene Expression and Subcellular Protein Expression Pattern of Protein Kinase C Isoforms in Curcumin-treated Human Hepatocellular Carcinoma Hep 3B Cells

Curcumin, a yellow component of turmeric or curry powder, has been demonstrated to exhibit anti-carcinogenic effects in vitro , in vivo , and in human clinical trials. One of its molecular targets is protein kinase C (PKC) which has been reported to play essential roles in apoptosis, cell proliferat...

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Published in:Plant foods for human nutrition (Dordrecht) 2011-06, Vol.66 (2), p.136-142
Main Authors: Kao, Hsin-Hsin, Wu, Chao-Jung, Won, Shen-Jeu, Shin, Jyh-Wei, Liu, Hsiao-Sheng, Su, Chun-Li
Format: Article
Language:English
Subjects:
Anticarcinogenic Agents - pharmacology
Biological and medical sciences
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - enzymology
Cell Line, Tumor
Cell Membrane - drug effects
Cell Membrane - enzymology
Cell Nucleus - drug effects
Cell Nucleus - enzymology
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Curcuma longa
Curcumin - pharmacology
Cytosol - drug effects
Cytosol - enzymology
Ecology
Food industries
Food Science
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
Isoenzymes - drug effects
Isoenzymes - genetics
Isoenzymes - metabolism
Liver Neoplasms - drug therapy
Liver Neoplasms - enzymology
Nutrition
Oligonucleotide Array Sequence Analysis
Original Paper
Plant Physiology
Protein Kinase C - drug effects
Protein Kinase C - genetics
Protein Kinase C - metabolism
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Summary:Curcumin, a yellow component of turmeric or curry powder, has been demonstrated to exhibit anti-carcinogenic effects in vitro , in vivo , and in human clinical trials. One of its molecular targets is protein kinase C (PKC) which has been reported to play essential roles in apoptosis, cell proliferation, and carcinogenesis. In this study, PKC mRNA expression was significantly inhibited in curcumin-treated human hepatocellular carcinoma (HCC) Hep 3B cells identified using a kinase cDNA microarray. Furthermore, curcumin decreased total protein expression of all PKCs in a time-related manner by immunoblotting of whole cell lysates, nuclear, membrane, and cytosolic fractions. In cytosolic fraction, the expression of PKC-α was totally inhibited by curcumin. In contrast, the expression levels of PKC-ζ and -μ were dramatically increased. Increases in expression of PKC-δ and PKC-ζ in the membrane and nucleus, and PKC-ι in the membrane were detected. In summary, the changes in expression and distribution of subcellular PKC isoforms in curcumin-treated Hep 3B cells suggest possible PKC-associated anti-tumor mechanisms of curcumin and provide alternative therapies for human HCC.
ISSN:0921-9668
1573-9104
DOI:10.1007/s11130-011-0228-2