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Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina
•AP-2δ is expressed in one-third of retinal ganglion cells.•ST8SIA2 is induced by AP-2δ overexpression in chick retina.•ST8SIA2 is direct target of AP-2δ based on ChIP and gel shift analysis.•Increases in PSA levels upon AP-2δ overexpression may be due to ST8SIA2 activation. The AP-2δ transcription...
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| Published in: | FEBS letters 2014-03, Vol.588 (5), p.770-775 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c5102-392456e22c908194721993c3eb5c42748e0251651826cbaceb0ffde3e81da1893 |
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| cites | cdi_FETCH-LOGICAL-c5102-392456e22c908194721993c3eb5c42748e0251651826cbaceb0ffde3e81da1893 |
| container_end_page | 775 |
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| container_title | FEBS letters |
| container_volume | 588 |
| creator | Li, Xiaodong Persad, Amit R.L. Monckton, Elizabeth A. Godbout, Roseline |
| description | •AP-2δ is expressed in one-third of retinal ganglion cells.•ST8SIA2 is induced by AP-2δ overexpression in chick retina.•ST8SIA2 is direct target of AP-2δ based on ChIP and gel shift analysis.•Increases in PSA levels upon AP-2δ overexpression may be due to ST8SIA2 activation.
The AP-2δ transcription factor is restricted to a subset of retinal ganglion cells. Overexpression of AP-2δ in chick retina results in induction of polysialylated neural cell adhesion molecule (PSA-NCAM) accompanied by misrouting and bundling of ganglion cell axons. Two polysialyltransferases, ST8SIA2 and ST8SIA4, are responsible for polysialylation of NCAM. Here, we investigate the mechanism driving the increase in PSA-NCAM observed upon AP-2δ overexpression. We show that ST8SIA2 is induced by AP-2δ overexpression in chick retina. We use chromatin immunoprecipitation and gel shift assays to demonstrate direct interaction between AP-2δ and the ST8SIA2 promoter. We propose that up-regulation of ST8SIA2 upon AP-2δ overexpression in retina increases ectopic polysialylation of NCAM which in turn causes premature bundling of axons and alters axonal response to guidance cues. |
| doi_str_mv | 10.1016/j.febslet.2014.01.024 |
| format | article |
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The AP-2δ transcription factor is restricted to a subset of retinal ganglion cells. Overexpression of AP-2δ in chick retina results in induction of polysialylated neural cell adhesion molecule (PSA-NCAM) accompanied by misrouting and bundling of ganglion cell axons. Two polysialyltransferases, ST8SIA2 and ST8SIA4, are responsible for polysialylation of NCAM. Here, we investigate the mechanism driving the increase in PSA-NCAM observed upon AP-2δ overexpression. We show that ST8SIA2 is induced by AP-2δ overexpression in chick retina. We use chromatin immunoprecipitation and gel shift assays to demonstrate direct interaction between AP-2δ and the ST8SIA2 promoter. We propose that up-regulation of ST8SIA2 upon AP-2δ overexpression in retina increases ectopic polysialylation of NCAM which in turn causes premature bundling of axons and alters axonal response to guidance cues.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.01.024</identifier><identifier>PMID: 24462686</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Avian Proteins - genetics ; Avian Proteins - metabolism ; Axons - enzymology ; Base Sequence ; Binding Sites ; Chick Embryo ; Chromatin immunoprecipitation ; Gel shift ; Gene Expression Regulation, Enzymologic ; Polysialyltransferase ; Promoter Regions, Genetic ; Protein Binding ; PSA-NCAM ; Retina ; Retina - cytology ; Retina - enzymology ; Retinal Ganglion Cells - enzymology ; Sialyltransferases - genetics ; Sialyltransferases - metabolism ; ST8SIA2 ; Transcription Factor AP-2 - physiology</subject><ispartof>FEBS letters, 2014-03, Vol.588 (5), p.770-775</ispartof><rights>2014 Federation of European Biochemical Societies</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5102-392456e22c908194721993c3eb5c42748e0251651826cbaceb0ffde3e81da1893</citedby><cites>FETCH-LOGICAL-c5102-392456e22c908194721993c3eb5c42748e0251651826cbaceb0ffde3e81da1893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2014.01.024$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.febslet.2014.01.024$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,1424,27957,27958,45609,45610,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24462686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiaodong</creatorcontrib><creatorcontrib>Persad, Amit R.L.</creatorcontrib><creatorcontrib>Monckton, Elizabeth A.</creatorcontrib><creatorcontrib>Godbout, Roseline</creatorcontrib><title>Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•AP-2δ is expressed in one-third of retinal ganglion cells.•ST8SIA2 is induced by AP-2δ overexpression in chick retina.•ST8SIA2 is direct target of AP-2δ based on ChIP and gel shift analysis.•Increases in PSA levels upon AP-2δ overexpression may be due to ST8SIA2 activation.
The AP-2δ transcription factor is restricted to a subset of retinal ganglion cells. Overexpression of AP-2δ in chick retina results in induction of polysialylated neural cell adhesion molecule (PSA-NCAM) accompanied by misrouting and bundling of ganglion cell axons. Two polysialyltransferases, ST8SIA2 and ST8SIA4, are responsible for polysialylation of NCAM. Here, we investigate the mechanism driving the increase in PSA-NCAM observed upon AP-2δ overexpression. We show that ST8SIA2 is induced by AP-2δ overexpression in chick retina. We use chromatin immunoprecipitation and gel shift assays to demonstrate direct interaction between AP-2δ and the ST8SIA2 promoter. We propose that up-regulation of ST8SIA2 upon AP-2δ overexpression in retina increases ectopic polysialylation of NCAM which in turn causes premature bundling of axons and alters axonal response to guidance cues.</description><subject>Animals</subject><subject>Avian Proteins - genetics</subject><subject>Avian Proteins - metabolism</subject><subject>Axons - enzymology</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Chick Embryo</subject><subject>Chromatin immunoprecipitation</subject><subject>Gel shift</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Polysialyltransferase</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>PSA-NCAM</subject><subject>Retina</subject><subject>Retina - cytology</subject><subject>Retina - enzymology</subject><subject>Retinal Ganglion Cells - enzymology</subject><subject>Sialyltransferases - genetics</subject><subject>Sialyltransferases - metabolism</subject><subject>ST8SIA2</subject><subject>Transcription Factor AP-2 - physiology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkV1v0zAUhi0EYt3gJ4ByyU2Cv-NcoTJtbNKkIbVcW45zQl3cJNjutv57HLXjdruyLD_n9dH7IPSJ4IpgIr9uqx7a6CFVFBNeYVJhyt-gBVE1KxmX6i1a4PxSirphZ-g8xi3Od0Wa9-iMci6pVHKBNutghmiDm5Ibh6I3No2hWP4saQc-mSLA7703CWKRNlDA0xQgxpkc-2Ia_SE64w8-zSE9BBOhWK3V6nZJCzcUduPsnxyR3GA-oHe98RE-ns4L9Ov6an15U97d_7i9XN6VVhBMS9ZQLiRQahucd-U1JU3DLINWWE5rrgBTQaQgikrbGgst7vsOGCjSGaIadoG-HHOnMP7dQ0x656IF780A4z5qUovcTi5AvIyK3BcTspEZFUfUhjHGAL2egtuZcNAE69mH3uqTDz370Jjo7CPPfT59sW930P2fehaQgZsj8Og8HF6Xqq-vvtPVLHd2S3j2KhjNUd-OUZDrfXAQdLQOBgudC2CT7kb3wrb_AKOqs0k</recordid><startdate>20140303</startdate><enddate>20140303</enddate><creator>Li, Xiaodong</creator><creator>Persad, Amit R.L.</creator><creator>Monckton, Elizabeth A.</creator><creator>Godbout, Roseline</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20140303</creationdate><title>Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina</title><author>Li, Xiaodong ; Persad, Amit R.L. ; Monckton, Elizabeth A. ; Godbout, Roseline</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5102-392456e22c908194721993c3eb5c42748e0251651826cbaceb0ffde3e81da1893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Avian Proteins - genetics</topic><topic>Avian Proteins - metabolism</topic><topic>Axons - enzymology</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Chick Embryo</topic><topic>Chromatin immunoprecipitation</topic><topic>Gel shift</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Polysialyltransferase</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>PSA-NCAM</topic><topic>Retina</topic><topic>Retina - cytology</topic><topic>Retina - enzymology</topic><topic>Retinal Ganglion Cells - enzymology</topic><topic>Sialyltransferases - genetics</topic><topic>Sialyltransferases - metabolism</topic><topic>ST8SIA2</topic><topic>Transcription Factor AP-2 - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiaodong</creatorcontrib><creatorcontrib>Persad, Amit R.L.</creatorcontrib><creatorcontrib>Monckton, Elizabeth A.</creatorcontrib><creatorcontrib>Godbout, Roseline</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiaodong</au><au>Persad, Amit R.L.</au><au>Monckton, Elizabeth A.</au><au>Godbout, Roseline</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-03-03</date><risdate>2014</risdate><volume>588</volume><issue>5</issue><spage>770</spage><epage>775</epage><pages>770-775</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>•AP-2δ is expressed in one-third of retinal ganglion cells.•ST8SIA2 is induced by AP-2δ overexpression in chick retina.•ST8SIA2 is direct target of AP-2δ based on ChIP and gel shift analysis.•Increases in PSA levels upon AP-2δ overexpression may be due to ST8SIA2 activation.
The AP-2δ transcription factor is restricted to a subset of retinal ganglion cells. Overexpression of AP-2δ in chick retina results in induction of polysialylated neural cell adhesion molecule (PSA-NCAM) accompanied by misrouting and bundling of ganglion cell axons. Two polysialyltransferases, ST8SIA2 and ST8SIA4, are responsible for polysialylation of NCAM. Here, we investigate the mechanism driving the increase in PSA-NCAM observed upon AP-2δ overexpression. We show that ST8SIA2 is induced by AP-2δ overexpression in chick retina. We use chromatin immunoprecipitation and gel shift assays to demonstrate direct interaction between AP-2δ and the ST8SIA2 promoter. We propose that up-regulation of ST8SIA2 upon AP-2δ overexpression in retina increases ectopic polysialylation of NCAM which in turn causes premature bundling of axons and alters axonal response to guidance cues.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>24462686</pmid><doi>10.1016/j.febslet.2014.01.024</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2014-03, Vol.588 (5), p.770-775 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS; Wiley Online Library |
| subjects | Animals Avian Proteins - genetics Avian Proteins - metabolism Axons - enzymology Base Sequence Binding Sites Chick Embryo Chromatin immunoprecipitation Gel shift Gene Expression Regulation, Enzymologic Polysialyltransferase Promoter Regions, Genetic Protein Binding PSA-NCAM Retina Retina - cytology Retina - enzymology Retinal Ganglion Cells - enzymology Sialyltransferases - genetics Sialyltransferases - metabolism ST8SIA2 Transcription Factor AP-2 - physiology |
| title | Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina |
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