Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina

•AP-2δ is expressed in one-third of retinal ganglion cells.•ST8SIA2 is induced by AP-2δ overexpression in chick retina.•ST8SIA2 is direct target of AP-2δ based on ChIP and gel shift analysis.•Increases in PSA levels upon AP-2δ overexpression may be due to ST8SIA2 activation. The AP-2δ transcription...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2014-03, Vol.588 (5), p.770-775
Main Authors: Li, Xiaodong, Persad, Amit R.L., Monckton, Elizabeth A., Godbout, Roseline
Format: Article
Language:English
Subjects:
Animals
Avian Proteins - genetics
Avian Proteins - metabolism
Axons - enzymology
Base Sequence
Binding Sites
Chick Embryo
Chromatin immunoprecipitation
Gel shift
Gene Expression Regulation, Enzymologic
Polysialyltransferase
Promoter Regions, Genetic
Protein Binding
PSA-NCAM
Retina
Retina - cytology
Retina - enzymology
Retinal Ganglion Cells - enzymology
Sialyltransferases - genetics
Sialyltransferases - metabolism
ST8SIA2
Transcription Factor AP-2 - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•AP-2δ is expressed in one-third of retinal ganglion cells.•ST8SIA2 is induced by AP-2δ overexpression in chick retina.•ST8SIA2 is direct target of AP-2δ based on ChIP and gel shift analysis.•Increases in PSA levels upon AP-2δ overexpression may be due to ST8SIA2 activation. The AP-2δ transcription factor is restricted to a subset of retinal ganglion cells. Overexpression of AP-2δ in chick retina results in induction of polysialylated neural cell adhesion molecule (PSA-NCAM) accompanied by misrouting and bundling of ganglion cell axons. Two polysialyltransferases, ST8SIA2 and ST8SIA4, are responsible for polysialylation of NCAM. Here, we investigate the mechanism driving the increase in PSA-NCAM observed upon AP-2δ overexpression. We show that ST8SIA2 is induced by AP-2δ overexpression in chick retina. We use chromatin immunoprecipitation and gel shift assays to demonstrate direct interaction between AP-2δ and the ST8SIA2 promoter. We propose that up-regulation of ST8SIA2 upon AP-2δ overexpression in retina increases ectopic polysialylation of NCAM which in turn causes premature bundling of axons and alters axonal response to guidance cues.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2014.01.024