Genetic diversity and relationships among germplasm of Jatropha curcas L. revealed by RAPDs

Variation is the phenomenon where individuals of a population differ from each other. The variation or diversity can have morphological manifestation or genetic basis. Individual identification based on morphological record is the most common practice in Jatropha. Therefore, in order to find a prope...

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Bibliographic Details
Published in:Trees (Berlin, West) West), 2009-10, Vol.23 (5), p.1075-1079
Main Authors: Kumar, R. V, Tripathi, Yogendra K, Shukla, Parul, Ahlawat, S. P, Gupta, V. K
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Cluster analysis
Forestry
Genetic diversity
Germplasm
Jatropha curcas
Life Sciences
Original Paper
Plant Anatomy/Development
Plant Pathology
Plant Physiology
Plant Sciences
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Summary:Variation is the phenomenon where individuals of a population differ from each other. The variation or diversity can have morphological manifestation or genetic basis. Individual identification based on morphological record is the most common practice in Jatropha. Therefore, in order to find a proper method for estimation of genetic diversity and genetic relationships among different germplasm of Jatropha curcas L., random amplified polymorphic DNA (RAPD) technique based on polymerase chain reaction (PCR) was used for described purpose. Out of 55 decamer primers tested, 26 primers produced good amplification products. A total of 6,011 amplification products were scored from which only 1,859 bands (30.92%) were found to be polymorphic and the size of bands ranged from 300 to 2,500 bp. Unweighted pair group method using arithmetic average cluster analysis revealed clear genetic difference among J. curcas germplasm. The scientific data presented in this study suggests that RAPD-PCR could be used as a valuable tool for estimation of genetic diversity and genetic relationship among germplasm of J. curcas L.
ISSN:0931-1890
1432-2285
DOI:10.1007/s00468-009-0350-z