Loading…
Identification of eight chromosomes and a microsatellite marker on 1as associated with QTL for grain weight in bread wheat
The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested tha...
Saved in:
Published in: | Theoretical and applied genetics 2000-06, Vol.100 (8), p.1290-1294 |
---|---|
Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c384t-2ccbad844bb90da56216686b63d4e738725d960e5bcc7463961671ee0887f363 |
---|---|
cites | |
container_end_page | 1294 |
container_issue | 8 |
container_start_page | 1290 |
container_title | Theoretical and applied genetics |
container_volume | 100 |
creator | VARSHNEY, R. K PRASAD, M ROY, J. K KUMAR, N HARJIT-SINGH DHALIWAL, H. S BALYAN, H. S GUPTA, P. K |
description | The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested that 8 chromosomes (1A, 1D, 2B, 4B, 5B, 6B, 7A and 7D) carried QTLs controlling GW, with only 3 of these (1A, 2B, 7A) carrying alleles for high GW. To tag the QTLs present on these chromosomes, we crossed the genotype RS111 with high GW (56.83 g) with the genotype Chinese Spring (CS) with low GW (23.74 g) and obtained 100 RILs. These RILs showed normal distribution for GW. The parental genotypes were analysed with as many as 346 STMS primer pairs for detection of polymorphism. Of these, 267 primer pairs gave scorable amplification products, 63 of which detected polymorphism between the parents. Using each of these 63 primer pairs, we carried out bulked segregant analysis on RILs representing two extremes of the distribution. One primer pair (WMC333) showed an association of the marker locus Xwmc333 with grain weight. This was confirmed through selective genotyping, and the co-segregation data on molecular marker locus Xwmc333 and GW were analysed following a single marker linear regression approach. Significant regression suggested linkage between Xwmc333 and a QTL for GW. The results showed that the above QTL accounted for 15.09% of the variation for GW between the parents. The marker has been located on chromosome arm 1AS, and QTL was designated QGw1.ccsu-1A. |
doi_str_mv | 10.1007/s001220051437 |
format | article |
fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_17532892</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A301556808</galeid><sourcerecordid>A301556808</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-2ccbad844bb90da56216686b63d4e738725d960e5bcc7463961671ee0887f363</originalsourceid><addsrcrecordid>eNpd0c2LEzEUAPAgCtbq0XtQETyMvnxO5rgsqxYKovYe3mQybdaZyZqkrPrXm9IFqacXyO893gchLxm8ZwDthwzAOAdQTIr2EVnVwBvOJX9MVgASGtUq_pQ8y_kWALgCsSJ_NoNfShiDwxLiQuNIfdgfCnWHFOeY4-wzxWWgSOfgUsxY_DSF4umM6YdPtOYwrCTn6EL9HOh9KAf6dbelY0x0nzAs9P5cs7765LGSg8fynDwZccr-xUNck93Hm93152b75dPm-mrbOGFkabhzPQ5Gyr7vYEClOdPa6F6LQfpWmJarodPgVe9cK7XoNNMt8x6MaUehxZq8PZe9S_Hn0edi55BdHQIXH4_ZslYJbjpe4av_4G08pqW2Zo1UhrGuyjV5fUZ7nLwNyxhLQneqaK8EMKW0AVPVuwvl4lL8r7LHY8528_3bpW3O9rTdnPxo71Koy_1tGdjTXe3FXat_89AmZofTmHBxIf9LksA704m_QcOfZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>845811975</pqid></control><display><type>article</type><title>Identification of eight chromosomes and a microsatellite marker on 1as associated with QTL for grain weight in bread wheat</title><source>Springer Link</source><creator>VARSHNEY, R. K ; PRASAD, M ; ROY, J. K ; KUMAR, N ; HARJIT-SINGH ; DHALIWAL, H. S ; BALYAN, H. S ; GUPTA, P. K</creator><creatorcontrib>VARSHNEY, R. K ; PRASAD, M ; ROY, J. K ; KUMAR, N ; HARJIT-SINGH ; DHALIWAL, H. S ; BALYAN, H. S ; GUPTA, P. K</creatorcontrib><description>The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested that 8 chromosomes (1A, 1D, 2B, 4B, 5B, 6B, 7A and 7D) carried QTLs controlling GW, with only 3 of these (1A, 2B, 7A) carrying alleles for high GW. To tag the QTLs present on these chromosomes, we crossed the genotype RS111 with high GW (56.83 g) with the genotype Chinese Spring (CS) with low GW (23.74 g) and obtained 100 RILs. These RILs showed normal distribution for GW. The parental genotypes were analysed with as many as 346 STMS primer pairs for detection of polymorphism. Of these, 267 primer pairs gave scorable amplification products, 63 of which detected polymorphism between the parents. Using each of these 63 primer pairs, we carried out bulked segregant analysis on RILs representing two extremes of the distribution. One primer pair (WMC333) showed an association of the marker locus Xwmc333 with grain weight. This was confirmed through selective genotyping, and the co-segregation data on molecular marker locus Xwmc333 and GW were analysed following a single marker linear regression approach. Significant regression suggested linkage between Xwmc333 and a QTL for GW. The results showed that the above QTL accounted for 15.09% of the variation for GW between the parents. The marker has been located on chromosome arm 1AS, and QTL was designated QGw1.ccsu-1A.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220051437</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Chromosomes ; Fundamental and applied biological sciences. Psychology ; Genetic aspects ; Genetics and breeding of economic plants ; Genotype & phenotype ; Physiological aspects ; Plant genetics ; Polymerase chain reaction ; Polymorphism ; Quantitative trait loci ; Regression analysis ; Triticum aestivum ; Varietal selection. Specialized plant breeding, plant breeding aims ; Wheat ; Yield, quality, earliness, varia</subject><ispartof>Theoretical and applied genetics, 2000-06, Vol.100 (8), p.1290-1294</ispartof><rights>2000 INIST-CNRS</rights><rights>COPYRIGHT 2000 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-2ccbad844bb90da56216686b63d4e738725d960e5bcc7463961671ee0887f363</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1402989$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>VARSHNEY, R. K</creatorcontrib><creatorcontrib>PRASAD, M</creatorcontrib><creatorcontrib>ROY, J. K</creatorcontrib><creatorcontrib>KUMAR, N</creatorcontrib><creatorcontrib>HARJIT-SINGH</creatorcontrib><creatorcontrib>DHALIWAL, H. S</creatorcontrib><creatorcontrib>BALYAN, H. S</creatorcontrib><creatorcontrib>GUPTA, P. K</creatorcontrib><title>Identification of eight chromosomes and a microsatellite marker on 1as associated with QTL for grain weight in bread wheat</title><title>Theoretical and applied genetics</title><description>The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested that 8 chromosomes (1A, 1D, 2B, 4B, 5B, 6B, 7A and 7D) carried QTLs controlling GW, with only 3 of these (1A, 2B, 7A) carrying alleles for high GW. To tag the QTLs present on these chromosomes, we crossed the genotype RS111 with high GW (56.83 g) with the genotype Chinese Spring (CS) with low GW (23.74 g) and obtained 100 RILs. These RILs showed normal distribution for GW. The parental genotypes were analysed with as many as 346 STMS primer pairs for detection of polymorphism. Of these, 267 primer pairs gave scorable amplification products, 63 of which detected polymorphism between the parents. Using each of these 63 primer pairs, we carried out bulked segregant analysis on RILs representing two extremes of the distribution. One primer pair (WMC333) showed an association of the marker locus Xwmc333 with grain weight. This was confirmed through selective genotyping, and the co-segregation data on molecular marker locus Xwmc333 and GW were analysed following a single marker linear regression approach. Significant regression suggested linkage between Xwmc333 and a QTL for GW. The results showed that the above QTL accounted for 15.09% of the variation for GW between the parents. The marker has been located on chromosome arm 1AS, and QTL was designated QGw1.ccsu-1A.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Chromosomes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic aspects</subject><subject>Genetics and breeding of economic plants</subject><subject>Genotype & phenotype</subject><subject>Physiological aspects</subject><subject>Plant genetics</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Quantitative trait loci</subject><subject>Regression analysis</subject><subject>Triticum aestivum</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>Wheat</subject><subject>Yield, quality, earliness, varia</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpd0c2LEzEUAPAgCtbq0XtQETyMvnxO5rgsqxYKovYe3mQybdaZyZqkrPrXm9IFqacXyO893gchLxm8ZwDthwzAOAdQTIr2EVnVwBvOJX9MVgASGtUq_pQ8y_kWALgCsSJ_NoNfShiDwxLiQuNIfdgfCnWHFOeY4-wzxWWgSOfgUsxY_DSF4umM6YdPtOYwrCTn6EL9HOh9KAf6dbelY0x0nzAs9P5cs7765LGSg8fynDwZccr-xUNck93Hm93152b75dPm-mrbOGFkabhzPQ5Gyr7vYEClOdPa6F6LQfpWmJarodPgVe9cK7XoNNMt8x6MaUehxZq8PZe9S_Hn0edi55BdHQIXH4_ZslYJbjpe4av_4G08pqW2Zo1UhrGuyjV5fUZ7nLwNyxhLQneqaK8EMKW0AVPVuwvl4lL8r7LHY8528_3bpW3O9rTdnPxo71Koy_1tGdjTXe3FXat_89AmZofTmHBxIf9LksA704m_QcOfZw</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>VARSHNEY, R. K</creator><creator>PRASAD, M</creator><creator>ROY, J. K</creator><creator>KUMAR, N</creator><creator>HARJIT-SINGH</creator><creator>DHALIWAL, H. S</creator><creator>BALYAN, H. S</creator><creator>GUPTA, P. K</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope></search><sort><creationdate>20000601</creationdate><title>Identification of eight chromosomes and a microsatellite marker on 1as associated with QTL for grain weight in bread wheat</title><author>VARSHNEY, R. K ; PRASAD, M ; ROY, J. K ; KUMAR, N ; HARJIT-SINGH ; DHALIWAL, H. S ; BALYAN, H. S ; GUPTA, P. K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-2ccbad844bb90da56216686b63d4e738725d960e5bcc7463961671ee0887f363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Chromosomes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic aspects</topic><topic>Genetics and breeding of economic plants</topic><topic>Genotype & phenotype</topic><topic>Physiological aspects</topic><topic>Plant genetics</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Quantitative trait loci</topic><topic>Regression analysis</topic><topic>Triticum aestivum</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>Wheat</topic><topic>Yield, quality, earliness, varia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VARSHNEY, R. K</creatorcontrib><creatorcontrib>PRASAD, M</creatorcontrib><creatorcontrib>ROY, J. K</creatorcontrib><creatorcontrib>KUMAR, N</creatorcontrib><creatorcontrib>HARJIT-SINGH</creatorcontrib><creatorcontrib>DHALIWAL, H. S</creatorcontrib><creatorcontrib>BALYAN, H. S</creatorcontrib><creatorcontrib>GUPTA, P. K</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Gale in Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VARSHNEY, R. K</au><au>PRASAD, M</au><au>ROY, J. K</au><au>KUMAR, N</au><au>HARJIT-SINGH</au><au>DHALIWAL, H. S</au><au>BALYAN, H. S</au><au>GUPTA, P. K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of eight chromosomes and a microsatellite marker on 1as associated with QTL for grain weight in bread wheat</atitle><jtitle>Theoretical and applied genetics</jtitle><date>2000-06-01</date><risdate>2000</risdate><volume>100</volume><issue>8</issue><spage>1290</spage><epage>1294</epage><pages>1290-1294</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested that 8 chromosomes (1A, 1D, 2B, 4B, 5B, 6B, 7A and 7D) carried QTLs controlling GW, with only 3 of these (1A, 2B, 7A) carrying alleles for high GW. To tag the QTLs present on these chromosomes, we crossed the genotype RS111 with high GW (56.83 g) with the genotype Chinese Spring (CS) with low GW (23.74 g) and obtained 100 RILs. These RILs showed normal distribution for GW. The parental genotypes were analysed with as many as 346 STMS primer pairs for detection of polymorphism. Of these, 267 primer pairs gave scorable amplification products, 63 of which detected polymorphism between the parents. Using each of these 63 primer pairs, we carried out bulked segregant analysis on RILs representing two extremes of the distribution. One primer pair (WMC333) showed an association of the marker locus Xwmc333 with grain weight. This was confirmed through selective genotyping, and the co-segregation data on molecular marker locus Xwmc333 and GW were analysed following a single marker linear regression approach. Significant regression suggested linkage between Xwmc333 and a QTL for GW. The results showed that the above QTL accounted for 15.09% of the variation for GW between the parents. The marker has been located on chromosome arm 1AS, and QTL was designated QGw1.ccsu-1A.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220051437</doi><tpages>5</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0040-5752 |
ispartof | Theoretical and applied genetics, 2000-06, Vol.100 (8), p.1290-1294 |
issn | 0040-5752 1432-2242 |
language | eng |
recordid | cdi_proquest_miscellaneous_17532892 |
source | Springer Link |
subjects | Agronomy. Soil science and plant productions Biological and medical sciences Chromosomes Fundamental and applied biological sciences. Psychology Genetic aspects Genetics and breeding of economic plants Genotype & phenotype Physiological aspects Plant genetics Polymerase chain reaction Polymorphism Quantitative trait loci Regression analysis Triticum aestivum Varietal selection. Specialized plant breeding, plant breeding aims Wheat Yield, quality, earliness, varia |
title | Identification of eight chromosomes and a microsatellite marker on 1as associated with QTL for grain weight in bread wheat |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T00%3A35%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20eight%20chromosomes%20and%20a%20microsatellite%20marker%20on%201as%20associated%20with%20QTL%20for%20grain%20weight%20in%20bread%20wheat&rft.jtitle=Theoretical%20and%20applied%20genetics&rft.au=VARSHNEY,%20R.%20K&rft.date=2000-06-01&rft.volume=100&rft.issue=8&rft.spage=1290&rft.epage=1294&rft.pages=1290-1294&rft.issn=0040-5752&rft.eissn=1432-2242&rft.coden=THAGA6&rft_id=info:doi/10.1007/s001220051437&rft_dat=%3Cgale_proqu%3EA301556808%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c384t-2ccbad844bb90da56216686b63d4e738725d960e5bcc7463961671ee0887f363%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=845811975&rft_id=info:pmid/&rft_galeid=A301556808&rfr_iscdi=true |