Identification of eight chromosomes and a microsatellite marker on 1as associated with QTL for grain weight in bread wheat

The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested tha...

Full description

Saved in:
Bibliographic Details
Published in:Theoretical and applied genetics 2000-06, Vol.100 (8), p.1290-1294
Main Authors: VARSHNEY, R. K, PRASAD, M, ROY, J. K, KUMAR, N, HARJIT-SINGH, DHALIWAL, H. S, BALYAN, H. S, GUPTA, P. K
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Chromosomes
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetics and breeding of economic plants
Genotype & phenotype
Physiological aspects
Plant genetics
Polymerase chain reaction
Polymorphism
Quantitative trait loci
Regression analysis
Triticum aestivum
Varietal selection. Specialized plant breeding, plant breeding aims
Wheat
Yield, quality, earliness, varia
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested that 8 chromosomes (1A, 1D, 2B, 4B, 5B, 6B, 7A and 7D) carried QTLs controlling GW, with only 3 of these (1A, 2B, 7A) carrying alleles for high GW. To tag the QTLs present on these chromosomes, we crossed the genotype RS111 with high GW (56.83 g) with the genotype Chinese Spring (CS) with low GW (23.74 g) and obtained 100 RILs. These RILs showed normal distribution for GW. The parental genotypes were analysed with as many as 346 STMS primer pairs for detection of polymorphism. Of these, 267 primer pairs gave scorable amplification products, 63 of which detected polymorphism between the parents. Using each of these 63 primer pairs, we carried out bulked segregant analysis on RILs representing two extremes of the distribution. One primer pair (WMC333) showed an association of the marker locus Xwmc333 with grain weight. This was confirmed through selective genotyping, and the co-segregation data on molecular marker locus Xwmc333 and GW were analysed following a single marker linear regression approach. Significant regression suggested linkage between Xwmc333 and a QTL for GW. The results showed that the above QTL accounted for 15.09% of the variation for GW between the parents. The marker has been located on chromosome arm 1AS, and QTL was designated QGw1.ccsu-1A.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220051437