Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

The objective of the current article was to compare the quality of the healing process after hysterorrhaphy with catgut and chitosan suture wires via macroscopic, histochemical, and immunohistochemical evaluations. Seven ewes were submitted to a video-assisted laparotomy. A hysterectomy was performe...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2016-01, Vol.104 (1), p.50-57
Main Authors: Huaixan, Lucio N., Arruda, Silvana S. B., Leonardo, André S., Viana, Janiny C., Barreto-Vianna, André R. C., Ximenes, Fábio H. B., Agreste, Fernanda R., Godoy, Roberta F., Lima, Eduardo M. M.
Format: Article
Language:English
Subjects:
Adhesion
Animals
biomaterials
Biomedical materials
biopolymers
Blood vessels
Catgut
Chitosan
Collagen
Collagen (type I)
Collagen (type III)
Collagen Type I - metabolism
Collagen Type III - metabolism
Connective tissues
Female
Healing
Horns
Hysterectomy
Immunohistochemistry
Laparotomy
Materials research
Materials science
physical properties
Sheep
Sutures
tissue engineering
uterine healing
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - metabolism
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Summary:The objective of the current article was to compare the quality of the healing process after hysterorrhaphy with catgut and chitosan suture wires via macroscopic, histochemical, and immunohistochemical evaluations. Seven ewes were submitted to a video-assisted laparotomy. A hysterectomy was performed with subsequent catgut and chitosan hysterorrhaphy in both horns. Then, macroscopic evaluation and biopsies of the hysterorrhaphy and control areas were carried out after 30 days. Although postoperative adhesion was observed in five animals of the catgut group, no adhesions were detected in the chitosan one. Additionally, there was a significant difference in increasing collagen type III and connective tissue for the chitosan group when compared to the control and catgut groups. A larger amount of blood vessels was also observed in the control group in comparison with the catgut and chitosan groups. Collagen type I and vascular endothelial growth factor-A (VEGF-A) were significantly different among groups. Thus, chitosan promoted a preventive effect on postoperative adhesion formation in the hysterorrhaphy areas, with increasing collagen type III deposition during tissue mending, which granted an enhanced healing process.
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.33369