Plasma glutathione reductase activity and prognosis of septic shock

Abstract Background Our aim was to investigate whether plasma glutathione reductase (GR) activity is well correlated with the erythrocyte-reduced glutathione (GSH)/glutathione disulfide (GSSG) ratio and is associated with the mortality of septic shock. Materials and methods This study was conducted...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of surgical research 2016-01, Vol.200 (1), p.298-307
Main Authors: Kim, Jae Seong, MD, Kwon, Woon Yong, MD, PhD, Suh, Gil Joon, MD, PhD, Kim, Kyung Su, MD, PhD, Jung, Yoon Sun, MD, Kim, Sung Hee, MSc, Lee, So Eun, MD
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Animals
Biomarkers - blood
Blotting, Western
Erythrocytes - metabolism
Female
Glutathione
Glutathione - blood
Glutathione Disulfide - blood
Glutathione reductase
Glutathione Reductase - blood
Humans
Logistic Models
Male
Middle Aged
Mortality
Oxidation reduction
Oxidative stress
Prognosis
Rats
Rats, Sprague-Dawley
Septic shock
Shock, Septic - blood
Shock, Septic - enzymology
Shock, Septic - mortality
Surgery
Survival Analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Background Our aim was to investigate whether plasma glutathione reductase (GR) activity is well correlated with the erythrocyte-reduced glutathione (GSH)/glutathione disulfide (GSSG) ratio and is associated with the mortality of septic shock. Materials and methods This study was conducted on male Sprague–Dawley rats and patients admitted to the intensive care unit with septic shock. To induce endotoxemia in rats, vehicle or lipopolysaccharide (LPS) at dosages of 5 or 10 mg/kg were injected into a tail vein. Animals were then euthanized 6 h post-LPS. Based on the 28-d mortality, the enrolled patients were divided into the survivors and nonsurvivors. We obtained blood samples from patients at admission (0 h) and 24 h after admission to the intensive care unit. Results In endotoxemic rats, the erythrocyte GSH/GSSG ratio, erythrocyte GR activity, and plasma GR activity in the 10 mg/kg of LPS group were lower than those in the sham and 5 mg/kg of LPS groups. In patients with septic shock, decrease in plasma GR activity at 24 h was independently associated with an increase in 28-d mortality (odds ratio, 0.828; 95% confidence interval, 0.690–0.992, P  = 0.041). Plasma GR activity was correlated with erythrocyte GR activity (Spearman ρ  = 0.549, P  
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2015.07.044