Cloning, expression and nucleotide sequences of two xylanase genes from Paenibacillus sp

Two genes, xynA and xynB, encoding xylanases from Paenibacillus sp. KCTC 8848P were cloned and expressed in Escherichia coli, and their nucleotide sequences were determined. The xylanases of E. coli transformants were released into the extracellular culture fluid in the absence of xylan. The structu...

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Bibliographic Details
Published in:Biotechnology letters 2000-03, Vol.22 (5), p.387-392
Main Authors: LEE, H.-J, SHIN, D.-J, NAM CHUL CHO, KIM, H.-O, SHIN, S.-Y, IM, S.-Y, LEE, H. B, CHUN, S.-B, SUK BAI
Format: Article
Language:English
Subjects:
Amino acids
Bacteria
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
E coli
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genes
Genetics
Mission oriented research
Paenibacillus
xynA gene
xynB gene
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Summary:Two genes, xynA and xynB, encoding xylanases from Paenibacillus sp. KCTC 8848P were cloned and expressed in Escherichia coli, and their nucleotide sequences were determined. The xylanases of E. coli transformants were released into the extracellular culture fluid in the absence of xylan. The structural gene of xynA 636 bp, encoded a protein of 212 amino acids, while the xynB gene consisted of 951 bp open reading frame for a protein of 317 amino acids. The amino acid sequence of the xynAgene showed 83% similarity to the xylanase of Aeromonas caviae, and belonged to the family 11 glycosyl hydrolases. The deduced amino acid sequence of the xynB gene, however, showed 51% similarity to the xylanase of Rhodothermus marinus, and belonged to the family 10 glycosyl hydrolases.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005676702533