Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity

During screening for biosurfactant-producing bacteria, a strain designated J36 T was isolated from oil-polluted site near Kaohsiung city located in southern Taiwan. Cells of this organism were Gram-negative rods motile by means of a single polar flagellum. Strain J36 T grew well in complex media und...

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Bibliographic Details
Published in:Systematic and applied microbiology 2005-03, Vol.28 (2), p.137-144
Main Authors: Chang, Jo-Shu, Chou, Cheng-Liang, Lin, Guang-Huey, Sheu, Shih-Yi, Chen, Wen-Ming
Format: Article
Language:English
Subjects:
Bacterial Proteins - analysis
Bacterial Typing Techniques
Bacteriology
Base Composition
Biological and medical sciences
Biosurfactant
Biotechnology
DNA, Bacterial - analysis
Fatty Acids - analysis
Fundamental and applied biological sciences. Psychology
Microbiology
Molecular Sequence Data
Petroleum
Phenotype
Phylogeny
Pseudoxanthomonas kaohsiungensis
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Surface Properties
Surface-Active Agents - metabolism
Systematics
Taiwan
Taxonomy
Water Microbiology
Water Pollution, Chemical
Xanthomonadaceae - chemistry
Xanthomonadaceae - classification
Xanthomonadaceae - genetics
Xanthomonadaceae - isolation & purification
Xanthomonadaceae - metabolism
Xanthomonas
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Summary:During screening for biosurfactant-producing bacteria, a strain designated J36 T was isolated from oil-polluted site near Kaohsiung city located in southern Taiwan. Cells of this organism were Gram-negative rods motile by means of a single polar flagellum. Strain J36 T grew well in complex media under optimum conditions of 35 °C and pH 7. The extracellular products of the strain expressed emulsification activity. During cultivation on olive oil as the sole carbon and energy source, the culture supernatant of strain J36 T reduced surface tension of the medium from 68 to 32.6 dyne/cm. The 16S rRNA gene sequence analysis indicates that strain J36 T is a member of Xanthomonas group within the γ-Proteobacteria. The organism belongs to the genus Pseudoxanthomonas and represents a novel species within this genus according to phylogenetic analysis of 16S rDNA sequences, DNA–DNA similarity data, whole-cell protein analysis, physiological and biochemical characteristics, as well as fatty acid compositions. The predominant cellular fatty acids of strain J36 T were 15:0 iso (about 26%), 17:1 iso ω9c (about 25%), and 15:0 anteiso (about 10%). Its DNA base ratio was 60.1 mol% G+C. We propose to classify strain J36 T (=BCRC 17375 T=LMG 22530 T) as Pseudoxanthomonas kaohsiungensis sp. nov.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2004.11.003