Caffeine protects against memory loss induced by high and non-anxiolytic dose of cannabidiol in adult zebrafish (Danio rerio)

Cannabidiol (CBD) has been investigated in a wide spectrum of clinical approaches due to its psychopharmacological properties. CBD has low affinity for cannabinoid neuroreceptors and agonistic properties to 5-HT receptors. An interaction between cannabinoid and purinergic receptor systems has been p...

Full description

Saved in:
Bibliographic Details
Published in:Pharmacology, biochemistry and behavior biochemistry and behavior, 2015-08, Vol.135, p.210-216
Main Authors: Nazario, Luiza Reali, Antonioli, Régis, Capiotti, Katiucia Marques, Hallak, Jaime Eduardo Cecílio, Zuardi, Antonio Waldo, Crippa, José Alexandre S., Bonan, Carla Denise, da Silva, Rosane Souza
Format: Article
Language:English
Subjects:
Adenosine
Adenosine A1 Receptor Antagonists - pharmacology
Adenosine A2 Receptor Antagonists - pharmacology
Animals
Anxiety
Avoidance Learning - drug effects
Behavior, Animal - drug effects
Caffeine
Caffeine - pharmacology
Cannabidiol
Cannabidiol - antagonists & inhibitors
Cannabidiol - toxicity
Central Nervous System Stimulants - pharmacology
Danio rerio
Dose-Response Relationship, Drug
Freshwater
Memory
Memory Disorders - chemically induced
Memory Disorders - drug therapy
Memory Disorders - psychology
Motor Activity - drug effects
Receptor, Adenosine A2A - drug effects
Zebrafish
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cannabidiol (CBD) has been investigated in a wide spectrum of clinical approaches due to its psychopharmacological properties. CBD has low affinity for cannabinoid neuroreceptors and agonistic properties to 5-HT receptors. An interaction between cannabinoid and purinergic receptor systems has been proposed. The purpose of this study is to evaluate CBD properties on memory behavioral and locomotor parameters and the effects of pre-treatment of adenosine receptor blockers on CBD impacts on memory using adult zebrafish. CBD (0.1, 0.5, 5, and 10mg/kg) was tested in the avoidance inhibitory paradigm and anxiety task. We analyzed the effect of a long-term caffeine pre-treatment (~20mg/L — four months). Also, acute block of adenosine receptors was performed in co-administration with CBD exposure in the memory assessment. CBD promoted an inverted U-shaped dose–response curve in the anxiety task; in the memory assessment, CBD in the dose of 5mg/Kg promoted the strongest effects without interfering with social and aggressive behavior. Caffeine treatment was able to prevent CBD (5mg/kg) effects on memory when CBD was given after the training session. CBD effects on memory were partially prevented by co-treatment with a specific A2A adenosine receptor antagonist when given prior to or after the training session, while CBD effects after the training session were fully prevented by adenosine A1 receptor antagonist. These results indicated that zebrafish have responses to CBD anxiolytic properties that are comparable to other animal models, and high doses changed memory retention in a way dependent on adenosine. •Zebrafish displays an inverted U dose–response curve to cannabidiol in the anxiety task.•CBD affects memory retention of zebrafish.•Long term caffeine treatment prevented CBD effects on memory.•Specific adenosine receptors antagonist prevented CBD effects on memory.
ISSN:0091-3057
1873-5177
DOI:10.1016/j.pbb.2015.06.008