Quality and fertilizing capacity of boar spermatozoa during liquid storage in extender supplemented with different antibiotics

The aim of the study was to determine the effect of antibiotics on quality parameters and fertilizing capacity of boar sperm during liquid preservation. In the first experiment, semen was diluted in an extender containing 200μg/mL of gentamicin as a control and diluted in a modified extenders: Ext I...

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Bibliographic Details
Published in:Animal reproduction science 2015-12, Vol.163, p.157-163
Main Authors: Bryła, Magdalena, Trzcińska, Monika
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Antibiotics
Apoptotic-like changes
Boar spermatozoa
Female
Fertility
Gilts
Insemination, Artificial - veterinary
Long-term storage
Male
Reproductive performance
Semen Analysis - veterinary
Semen Preservation
Spermatozoa - physiology
Swine
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Summary:The aim of the study was to determine the effect of antibiotics on quality parameters and fertilizing capacity of boar sperm during liquid preservation. In the first experiment, semen was diluted in an extender containing 200μg/mL of gentamicin as a control and diluted in a modified extenders: Ext I (contained 200μg/mL florfenicol), Ext II (contained 200μg/mL polymyxin B), Ext III (contained 100μg/mL gentamicin and 100μg/mL florfenicol) and Ext IV (contained 100μg/mL gentamicin and 100μg/mL polymyxin B). The semen was stored for ten days. Sperm quality was evaluated based on the motility (CASA; TM: total motility; PM: progressive motility), membrane integrity (YO-PRO-1/PI assay), mitochondrial activity (JC-1) and DNA integrity (TUNEL). The highest PM% (62.5±9.6) was observed in Ext III at Day 6 of storage. The highest sperm viability and mitochondrial transmembrane potential was noticed at the end of the storage period in Ext III. Long-term storage did not induce DNA fragmentation in the extenders analyzed. In the second experiment, semen diluted in the control extender and in the extender providing the highest quality spermatozoa on Day 10 (Ext III) was used for artificial insemination (AI) of synchronized gilts. Our studies showed that the highest reproductive performance of inseminated gilts (pregnant gilts: 97.0%, litter size: 11.4±1.2) occurred with Ext III semen dilution. The combination of 100μg/mL gentamicin and 100μg/mL florfenicol in the extender maintained sperm motility, membrane integrity and mitochondrial activity and enhanced the higher reproduction success.
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2015.11.005