Butylated hydroxytoluene can reduce oxidative stress and improve quality of frozen–thawed bull semen processed in lecithin and egg yolk based extenders

The aims of this study were to evaluate the effects of anti-oxidant butylated hydroxytoluene (BHT), when added at different concentrations into lecithin-based Bioxcell® (BX) and two egg-yolk-based; Tris (TY) and citrate (CE) semen extenders, on post-thaw bull sperm quality and oxidative stress. A to...

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Bibliographic Details
Published in:Animal reproduction science 2015-12, Vol.163, p.128-134
Main Authors: Khumran, A.M., Yimer, N., Rosnina, Y., Ariff, M.O., Wahid, H., Kaka, Asmatullah, Ebrahimi, M., Sarsaifi, K.
Format: Article
Language:English
Subjects:
Animals
Anti-oxidant
Antioxidants - pharmacology
Butylated hydroxytoluene
Butylated Hydroxytoluene - pharmacology
Cattle
Cryopreservation - veterinary
DNA Damage - drug effects
Egg Yolk
Extender
Freezing
Lecithins - pharmacology
Male
Oxidative stress
Oxidative Stress - drug effects
Semen Analysis - veterinary
Semen Preservation - methods
Semen Preservation - veterinary
Sperm Motility - drug effects
Sperm quality
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Summary:The aims of this study were to evaluate the effects of anti-oxidant butylated hydroxytoluene (BHT), when added at different concentrations into lecithin-based Bioxcell® (BX) and two egg-yolk-based; Tris (TY) and citrate (CE) semen extenders, on post-thaw bull sperm quality and oxidative stress. A total of 30 ejaculates from three bulls were collected using an electro ejaculator. Ejaculates were extended with one of the BX, TY and CE extenders, which contained different concentrations (0.0 – control, 0.5, 1.0, 1.5, 2.0 and 3.0mM/ml) of BHT. The extended semen samples were chilled to 4°C, and then frozen slowly to −196°C in 0.25ml straws before being stored in liquid nitrogen for 2 weeks. Results showed that supplementation of BHT improved (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2015.10.007