Aii20J, a wide-spectrum thermostable N-acylhomoserine lactonase from the marine bacterium Tenacibaculum sp. 20J, can quench AHL-mediated acid resistance in Escherichia coli

Acyl homoserine lactones (AHLs) are produced by many Gram-negative bacteria to coordinate gene expression in cellular density dependent mechanisms known as quorum sensing (QS). Since the disruption of the communication systems significantly reduces virulence, the inhibition of quorumsensing processe...

Full description

Saved in:

Bibliographic Details
Published in:	Applied microbiology and biotechnology 2015-11, Vol.99 (22), p.9523-9539
Main Authors:	Mayer, C, Romero, M, Muras, A, Otero, A
Format:	Article
Language:	eng
Subjects:	acid tolerance Acyl-Butyrolactones - metabolism Analysis Antimicrobial agents Aquaculture Bacillus Bacillus (bacteria) Bacillus - enzymology Bacillus - genetics Bacteria Bacterial infections beta-Lactamases - genetics Biofilms Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - metabolism cell viability Chymotrypsin - metabolism Cloning communications technology E coli Enzyme Stability Enzymes Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli - pathogenicity Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fatty acids Gene expression Gene Expression Regulation, Bacterial genes Gram-negative bacteria Health aspects homoserine Hydrogen-Ion Concentration Lactones Life Sciences Microbial Genetics and Genomics Microbial Viability Microbiology Pathogens proteinases Quorum Sensing Studies Tenacibaculum Tenacibaculum - enzymology Tenacibaculum - genetics thermal stability Trans-Activators - genetics Trans-Activators - metabolism Virulence Virulence - genetics
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Acyl homoserine lactones (AHLs) are produced by many Gram-negative bacteria to coordinate gene expression in cellular density dependent mechanisms known as quorum sensing (QS). Since the disruption of the communication systems significantly reduces virulence, the inhibition of quorumsensing processes or quorum quenching (QQ) represents an interesting anti-pathogenic strategy to control bacterial infections. Escherichia coli does not produce AHLs but possesses an orphan AHL receptor, SdiA, which is thought to be able to sense the QS signals produced by other bacteria and controls important traits as the expression of glutamate-dependent acid resistance mechanism, therefore constituting a putative target for QQ. A novel AHL-lactonase, named Aii20J, has been identified, cloned and over expressed from the marine bacterium Tenacibaculum sp. strain 20 J presenting a wide-spectrum QQ activity. The enzyme, belonging to the metallo-β-lactamase family, shares less than 31 % identity with the lactonase AiiA from Bacillus spp. Aii20J presents a much higher specific activity than the Bacillus enzyme, maintains its activity after incubation at 100 ºC for 10 minutes, is resistant to protease K and α-chymotrypsin, and is unaffected by wide ranges of pH. The addition of Aii20J (20 μg/mL) to cultures of E. coli K-12 to which OC6-HSL was added resulted in a significant reduction in cell viability in comparison with the acidresistant cultures derived from the presence of the signal. Results confirm the interaction between AHLs and SdiA in E. coli for the expression of virulence-related genes and reveal the potential use of Aii20J as anti-virulence strategy against important bacterial pathogens and in other biotechnological applications.
ISSN:	0175-7598 1432-0614