Dual effects of a lectin from the green seaweed Caulerpa cupressoides var. lycopodium on inflammatory mediators in classical models of inflammation

Objective Wide biotechnological investigations of only a limited number of seaweed lectins have been performed. We previously demonstrated the anti-nociceptive and anti-inflammatory effects of a lectin isolated from the green seaweed Caulerpa cupressoides var. lycopodium (CcL). Herein, we further st...

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Bibliographic Details
Published in:Inflammation research 2015-12, Vol.64 (12), p.971-982
Main Authors: de Queiroz, Ismael Nilo Lino, Quinderé, Ana Luíza Gomes, Rodrigues, José Ariévilo Gurgel, de Sousa Oliveira Vanderlei, Edfranck, Ribeiro, Natássia Albuquerque, da Conceição Rivanor, Renata Line, Ribeiro, Kátia Alves, Coura, Chistiane Oliveira, Pereira, Karuza Maria Alves, Chaves, Hellíada Vasconcelos, Bezerra, Mirna Marques, de Araújo, Ianna Wivianne Fernandes, Benevides, Norma Maria Barros
Format: Article
Language:English
Subjects:
Allergology
Animals
Anti-Inflammatory Agents - pharmacology
Biomedical and Life Sciences
Biomedicine
Carrageenan
Caulerpa - chemistry
Caulerpa cupressoides
Cytokines - biosynthesis
Dermatology
Edema - chemically induced
Edema - pathology
Foot - pathology
Histamine
Immunology
Inflammation - chemically induced
Inflammation - metabolism
Inflammation Mediators - metabolism
Lectins - pharmacology
Lycopodium
Male
Mucins - antagonists & inhibitors
Neurology
Neutrophil Infiltration - drug effects
Original Research Paper
Pharmacology/Toxicology
Rats
Rats, Wistar
Rheumatology
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Summary:Objective Wide biotechnological investigations of only a limited number of seaweed lectins have been performed. We previously demonstrated the anti-nociceptive and anti-inflammatory effects of a lectin isolated from the green seaweed Caulerpa cupressoides var. lycopodium (CcL). Herein, we further studied the mechanisms of action of CcL. Methods Classical acute inflammation models induced by different flogistic agents were used to evaluate the anti-inflammatory action of CcL. CcL was injected locally into the rat paw to verify a possible pro-inflammatory outcome. Results CcL (0.1, 1 or 10 mg/kg; i.v.) reduced the carrageenan-induced rat paw edema and neutrophilic infiltration, which was not altered by either mucin (inhibitor of CcL carbohydrate-binding site) or ZnPP-IX (specific HO-1 inhibitor). Immunohistochemical analyses showed that CcL (1 mg/kg) reduced the expression of the cytokines IL-1β, TNF-α, IL-6 and COX-2. CcL (0.1, 1 or 10 mg/kg) inhibited dextran, and CcL (1 mg/kg) inhibited histamine-induced rat paw edema. Both effects were reversed by mucin inhibition. CcL (1 mg/kg) was ineffective for the treatment of serotonin- and bradykinin-induced rat paw edema. When injected via the i.pl. route, CcL (10 mg/kg) elicited rat paw edema involving a wide range of mediators. Conclusions The anti-inflammatory action of CcL involves the inhibition of IL-1β, TNF-α, IL-6 and COX-2 expression and histamine H 1 receptors. When locally administered, CcL exerts pro-inflammatory actions.
ISSN:1023-3830
1420-908X
DOI:10.1007/s00011-015-0880-3