Deleterious impacts of a 900-MHz electromagnetic field on hippocampal pyramidal neurons of 8-week-old Sprague Dawley male rats

Abstract Children are at potential risk due to their intense use of mobile phones. We examined 8-week-old rats because this age of the rats is comparable with the preadolescent period in humans. The number of pyramidal neurons in the cornu ammonis of the Sprague Dawley male rat (8-weeks old, weighin...

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Published in:Brain research 2015-10, Vol.1624, p.232-238
Main Authors: Şahin, Arzu, Aslan, Ali, Baş, Orhan, İkinci, Ayşe, Özyılmaz, Cansu, Fikret Sönmez, Osman, Çolakoğlu, Serdar, Odacı, Ersan
Format: Article
Language:English
Subjects:
Animals
Cell Count
Cell Death - radiation effects
Cell phone
Children
Dose-Response Relationship, Radiation
Electromagnetic field
Electromagnetic Fields - adverse effects
Hippocampus
Hippocampus - cytology
Male
Male rat
Neurology
Pyramidal Cells - radiation effects
Pyramidal neuron
Rats
Rats, Sprague-Dawley
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Summary:Abstract Children are at potential risk due to their intense use of mobile phones. We examined 8-week-old rats because this age of the rats is comparable with the preadolescent period in humans. The number of pyramidal neurons in the cornu ammonis of the Sprague Dawley male rat (8-weeks old, weighing 180–250 g) hippocampus following exposure to a 900 MHz (MHz) electromagnetic field (EMF) were examined. The study consisted of control (CN-G), sham exposed (SHM-EG) and EMF exposed (EMF-EG) groups with 6 rats in each. The EMF-EG rats were exposed to 900 MHz EMF (1 h/day for 30 days) in an EMF jar. The SHM-EG rats were placed in the EMF jar but not exposed to the EMF (1 h/day for 30 days). The CN-G rats were not placed into the exposure jar and were not exposed to the EMF during the study period. All animals were sacrificed at the end of the experiment, and their brains were removed for histopathological and stereological analysis. The number of pyramidal neurons in the cornu ammonis of the hippocampus was estimated on Cresyl violet stained sections of the brain using the optical dissector counting technique. Histopathological evaluations were also performed on these sections. Histopathological observation showed abundant cells with abnormal, black or dark blue cytoplasm and shrunken morphology among the normal pyramidal neurons. The largest lateral ventricles were observed in the EMF-EG sections compared to those from the other groups. Stereological analyses showed that the total number of pyramidal neurons in the cornu ammonis of the EMF-EG rats was significantly lower than those in the CN-G ( p
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2015.07.042