Genetic analysis of circulating tumor cells in pancreatic cancer patients: A pilot study

Pancreatic cancer is one of the most aggressive malignant tumors, mainly due to an aggressive metastasis spreading. In recent years, circulating tumor cells became associated to tumor metastasis. Little is known about their expression profiles. The aim of this study was to develop a complete workflo...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2015-07, Vol.106 (1), p.7-14
Main Authors: Görner, Karin, Bachmann, Jeannine, Holzhauer, Claudia, Kirchner, Roland, Raba, Katharina, Fischer, Johannes C., Martignoni, Marc E., Schiemann, Matthias, Alunni-Fabbroni, Marianna
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Cell Line, Tumor
Cell Separation
Circulating tumor cells
Female
Flow Cytometry
Genetic profile
Humans
Male
Neoplastic Cells, Circulating - metabolism
Pancreatic cancer
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
Pilot Projects
RT-PCR
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Summary:Pancreatic cancer is one of the most aggressive malignant tumors, mainly due to an aggressive metastasis spreading. In recent years, circulating tumor cells became associated to tumor metastasis. Little is known about their expression profiles. The aim of this study was to develop a complete workflow making it possible to isolate circulating tumor cells from patients with pancreatic cancer and their genetic characterization. Results: We show that the proposed workflow offers a technical sensitivity and specificity high enough to detect and isolate single tumor cells. Moreover our approach makes feasible to genetically characterize single CTCs. Conclusions: Our work discloses a complete workflow to detect, count and genetically analyze individual CTCs isolated from blood samples. This method has a central impact on the early detection of metastasis development. The combination of cell quantification and genetic analysis provides the clinicians with a powerful tool not available so far.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2015.02.003